Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Fanyu Meng, Benjamin J. Cargile, Steven M. Patrie, Jeffrey R. Johnson, Thomas N. Corso, Shaun M. Mcloughlin, Neil L. Kelleher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages49-50
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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  • Cite this

    Meng, F., Cargile, B. J., Patrie, S. M., Johnson, J. R., Corso, T. N., Mcloughlin, S. M., & Kelleher, N. L. (2002). Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 49-50)