Hybridoma cells were prepared by immunizing mice with carboxylic derivatives of atrazine conjugate to bovine serum albumin. After the screening of culture supernatant of hybridomas, five cell lines producing monoclonal antibodies were established and 1.8-5.3 ml of ascitic fluid per mouse was obtained from each cell line. The protein A affinity purification yielded 0.35 0.65 mg per ml of ascitic fluid from each cell line. The characterization studies in terms of sensitivitiy and specificity indicate that MAb 2F9 and MAb 4B9 showed the best responses with atrazine and its group of ametryne and cyanazine, using microtiter plate coated with simazine derivative of 6-amino hexanoic acid; no cross-reactivity was shown with simazine and cyanuric chloride.
|Original language||English (US)|
|Number of pages||21|
|Journal||Journal of Immunoassay|
|Publication status||Published - 1999|
- Monoclonal antibody
ASJC Scopus subject areas