Production and characterization of monoclonal antibodies specific for the murine T cell receptor ζ chain

Nicolai S C van Oers, Soo Jeet Teh, Bryan A. Irving, Jacqueline Tiong, Arthur Weiss, Hung Sia Teh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The T cell receptor (TCR) comprises an antigen-specific αβ heterodimer non-covalently associated with the CD3 γδε{lunate} and TCR ζ subunits. Both the CD3 and TCR ζ subunits are proposed to be responsible for the intracellular signal-transduction events. We report here the production of eight monoclonal antibodies (mAbs) that bind in an ELISA assay to a 113 amino acid synthetic peptide corresponding to the cytoplasmic domain of TCR ζ. Western blot analysis of anti-CD8 precipitates of lysates of transfectants expressing chimeric CD8/ζ constructs encoding increasing COOH-terminal truncations of TCR ζ indicates that four of these mAbs recognized the region of TCR ζ chain comprising the last 29 COOH-terminal residues. Thus, this region of TCR ζ may encode an immunodominant epitope. Furthermore, one of these mAbs, G3, is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR ζ. The G3 mAb should be useful for elucidiating the structural and signalling characteristics of the TCR ζ chain.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalJournal of Immunological Methods
Volume170
Issue number2
DOIs
StatePublished - Apr 15 1994

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T-Cell Antigen Receptor
Monoclonal Antibodies
Immunodominant Epitopes
Tyrosine
Signal Transduction
Western Blotting
Enzyme-Linked Immunosorbent Assay
Amino Acids
Peptides

Keywords

  • Epitope mapping
  • Monoclonal antibody
  • T cell receptor ζ chain

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Production and characterization of monoclonal antibodies specific for the murine T cell receptor ζ chain. / van Oers, Nicolai S C; Teh, Soo Jeet; Irving, Bryan A.; Tiong, Jacqueline; Weiss, Arthur; Teh, Hung Sia.

In: Journal of Immunological Methods, Vol. 170, No. 2, 15.04.1994, p. 261-268.

Research output: Contribution to journalArticle

van Oers, Nicolai S C ; Teh, Soo Jeet ; Irving, Bryan A. ; Tiong, Jacqueline ; Weiss, Arthur ; Teh, Hung Sia. / Production and characterization of monoclonal antibodies specific for the murine T cell receptor ζ chain. In: Journal of Immunological Methods. 1994 ; Vol. 170, No. 2. pp. 261-268.
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