Production of autoantibodies against citrullinated antigens/peptides by human B cells

Maria F. Bellatin, Mei Han, Margarita Fallena, Lin Fan, Donglan Xia, Nancy Olsen, Valerie Branch, David Karp, Peter Stastny

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Autoantibodies against citrullinated protein Ags (ACPA) are associated with the development of rheumatoid arthritis (RA). This immune response against citrullinated protein Ags, which is thought to be facilitated by certain MHC HLA-DR alleles, is highly specific for this disease and has been speculated to be involved in the pathogenesis. We have previously studied cultures of B cells for the production of Abs against HLA Ags. The aim of the current study was to examine the role of B cells in the production of ACPA in patients with RA. Peripheral blood B cells from RA patients and healthy people were cultured with EL4-B5, a murine cell line expressing human CD40L, and with T cell factors to stimulate the in vitro production of Abs by B cells isolated from peripheral blood. ACPA were produced by cultured B cells from RA patients, as determined by reactivity to cyclic citrullinated peptide (CCP). The results showed that 22% of the healthy persons tested also had B cells that could produce ACPA. Patients with HLA-DR alleles carrying the RA-associated shared epitope appeared to have more B cells with autoimmune potential for CCP than those without such HLA alleles (odds ratio 8.1, p = 0.001). In healthy individuals, anti-CCP-producing B cells were also observed more frequently if the RA-associated MHC genes were present (odds ratio 8.0, p = 0.01). Analysis of B cells in cultures may shed light on the interaction of genetic and environmental factors in the development of RA.

Original languageEnglish (US)
Pages (from-to)3542-3550
Number of pages9
JournalJournal of Immunology
Volume188
Issue number7
DOIs
StatePublished - Apr 1 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Production of autoantibodies against citrullinated antigens/peptides by human B cells'. Together they form a unique fingerprint.

  • Cite this

    Bellatin, M. F., Han, M., Fallena, M., Fan, L., Xia, D., Olsen, N., Branch, V., Karp, D., & Stastny, P. (2012). Production of autoantibodies against citrullinated antigens/peptides by human B cells. Journal of Immunology, 188(7), 3542-3550. https://doi.org/10.4049/jimmunol.1100577