Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration

Steven J. Gray, Vivian W. Choi, Aravind Asokan, Rebecca A. Haberman, Thomas J. McCown, Richard Jude Samulski

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP.

Original languageEnglish (US)
Article number4.17
JournalCurrent Protocols in Neuroscience
Issue numberSUPPL.57
DOIs
StatePublished - Oct 1 2011

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Dependovirus
Transgenes
Adenoviridae
Rodentia
Spinal Cord
Viruses
Gene Expression
Brain
Infection
Research
Genes
In Vitro Techniques
Serogroup

Keywords

  • Adeno-associated virus
  • Brain
  • Gene delivery
  • Gene therapy
  • Purification
  • Serotypes
  • Spinal cord
  • Transduction
  • Transfection
  • Viral vectors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. / Gray, Steven J.; Choi, Vivian W.; Asokan, Aravind; Haberman, Rebecca A.; McCown, Thomas J.; Samulski, Richard Jude.

In: Current Protocols in Neuroscience, No. SUPPL.57, 4.17, 01.10.2011.

Research output: Contribution to journalArticle

Gray, Steven J. ; Choi, Vivian W. ; Asokan, Aravind ; Haberman, Rebecca A. ; McCown, Thomas J. ; Samulski, Richard Jude. / Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. In: Current Protocols in Neuroscience. 2011 ; No. SUPPL.57.
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