Production of transgenic rabbits, sheep and pigs by microinjection

Robert E Hammer, Vernon G. Pursel, Caird E. Rexroad, Robert J. Wall, Douglas J. Bolt, Karl M. Ebert, Richard D. Palmiter, Ralph L. Brinster

Research output: Contribution to journalArticle

673 Citations (Scopus)

Abstract

Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin1, Candida elegans2, Xenopus 3, Drosophila4,5 and mice6-11. Various genes have been successfully introduced into mice including constructs consisting of the mouse metallothionein-I (MT) promoter/regulator region fused to either the rat or human growth hormone (hGH) structural genes. Transgenic mice harbouring such genes commonly exhibit high, metal-inducible levels of the fusion messenger RNA in several organs, substantial quantities of the foreign growth hormone in serum and enhanced growth12,13. In addition, the gene is stably incorporated into the germ line, making the phenotype heritable. Because of the scientific importance and potential economic value of transgenic livestock containing foreign genes, we initiated studies on large animals by microinjecting the fusion gene, MT-hGH13, into the pronuclei or nuclei of eggs from superovulated rabbits, sheep and pigs. We report here integration of the gene in all three species and expression of the gene in transgenic rabbits and pigs.

Original languageEnglish (US)
Pages (from-to)680-683
Number of pages4
JournalNature
Volume315
Issue number6021
DOIs
StatePublished - 1985

Fingerprint

Microinjections
Sheep
Swine
Rabbits
Genes
Metallothionein
Human Growth Hormone
Gene Fusion
Livestock
Xenopus
Candida
Genetic Promoter Regions
Germ Cells
Oceans and Seas
Eggs
Transgenic Mice
Growth Hormone
Embryonic Structures
Metals
Economics

ASJC Scopus subject areas

  • General

Cite this

Hammer, R. E., Pursel, V. G., Rexroad, C. E., Wall, R. J., Bolt, D. J., Ebert, K. M., ... Brinster, R. L. (1985). Production of transgenic rabbits, sheep and pigs by microinjection. Nature, 315(6021), 680-683. https://doi.org/10.1038/315680a0

Production of transgenic rabbits, sheep and pigs by microinjection. / Hammer, Robert E; Pursel, Vernon G.; Rexroad, Caird E.; Wall, Robert J.; Bolt, Douglas J.; Ebert, Karl M.; Palmiter, Richard D.; Brinster, Ralph L.

In: Nature, Vol. 315, No. 6021, 1985, p. 680-683.

Research output: Contribution to journalArticle

Hammer, RE, Pursel, VG, Rexroad, CE, Wall, RJ, Bolt, DJ, Ebert, KM, Palmiter, RD & Brinster, RL 1985, 'Production of transgenic rabbits, sheep and pigs by microinjection', Nature, vol. 315, no. 6021, pp. 680-683. https://doi.org/10.1038/315680a0
Hammer RE, Pursel VG, Rexroad CE, Wall RJ, Bolt DJ, Ebert KM et al. Production of transgenic rabbits, sheep and pigs by microinjection. Nature. 1985;315(6021):680-683. https://doi.org/10.1038/315680a0
Hammer, Robert E ; Pursel, Vernon G. ; Rexroad, Caird E. ; Wall, Robert J. ; Bolt, Douglas J. ; Ebert, Karl M. ; Palmiter, Richard D. ; Brinster, Ralph L. / Production of transgenic rabbits, sheep and pigs by microinjection. In: Nature. 1985 ; Vol. 315, No. 6021. pp. 680-683.
@article{007e4c3c8b4d473d93170a81d775e3e4,
title = "Production of transgenic rabbits, sheep and pigs by microinjection",
abstract = "Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin1, Candida elegans2, Xenopus 3, Drosophila4,5 and mice6-11. Various genes have been successfully introduced into mice including constructs consisting of the mouse metallothionein-I (MT) promoter/regulator region fused to either the rat or human growth hormone (hGH) structural genes. Transgenic mice harbouring such genes commonly exhibit high, metal-inducible levels of the fusion messenger RNA in several organs, substantial quantities of the foreign growth hormone in serum and enhanced growth12,13. In addition, the gene is stably incorporated into the germ line, making the phenotype heritable. Because of the scientific importance and potential economic value of transgenic livestock containing foreign genes, we initiated studies on large animals by microinjecting the fusion gene, MT-hGH13, into the pronuclei or nuclei of eggs from superovulated rabbits, sheep and pigs. We report here integration of the gene in all three species and expression of the gene in transgenic rabbits and pigs.",
author = "Hammer, {Robert E} and Pursel, {Vernon G.} and Rexroad, {Caird E.} and Wall, {Robert J.} and Bolt, {Douglas J.} and Ebert, {Karl M.} and Palmiter, {Richard D.} and Brinster, {Ralph L.}",
year = "1985",
doi = "10.1038/315680a0",
language = "English (US)",
volume = "315",
pages = "680--683",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6021",

}

TY - JOUR

T1 - Production of transgenic rabbits, sheep and pigs by microinjection

AU - Hammer, Robert E

AU - Pursel, Vernon G.

AU - Rexroad, Caird E.

AU - Wall, Robert J.

AU - Bolt, Douglas J.

AU - Ebert, Karl M.

AU - Palmiter, Richard D.

AU - Brinster, Ralph L.

PY - 1985

Y1 - 1985

N2 - Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin1, Candida elegans2, Xenopus 3, Drosophila4,5 and mice6-11. Various genes have been successfully introduced into mice including constructs consisting of the mouse metallothionein-I (MT) promoter/regulator region fused to either the rat or human growth hormone (hGH) structural genes. Transgenic mice harbouring such genes commonly exhibit high, metal-inducible levels of the fusion messenger RNA in several organs, substantial quantities of the foreign growth hormone in serum and enhanced growth12,13. In addition, the gene is stably incorporated into the germ line, making the phenotype heritable. Because of the scientific importance and potential economic value of transgenic livestock containing foreign genes, we initiated studies on large animals by microinjecting the fusion gene, MT-hGH13, into the pronuclei or nuclei of eggs from superovulated rabbits, sheep and pigs. We report here integration of the gene in all three species and expression of the gene in transgenic rabbits and pigs.

AB - Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin1, Candida elegans2, Xenopus 3, Drosophila4,5 and mice6-11. Various genes have been successfully introduced into mice including constructs consisting of the mouse metallothionein-I (MT) promoter/regulator region fused to either the rat or human growth hormone (hGH) structural genes. Transgenic mice harbouring such genes commonly exhibit high, metal-inducible levels of the fusion messenger RNA in several organs, substantial quantities of the foreign growth hormone in serum and enhanced growth12,13. In addition, the gene is stably incorporated into the germ line, making the phenotype heritable. Because of the scientific importance and potential economic value of transgenic livestock containing foreign genes, we initiated studies on large animals by microinjecting the fusion gene, MT-hGH13, into the pronuclei or nuclei of eggs from superovulated rabbits, sheep and pigs. We report here integration of the gene in all three species and expression of the gene in transgenic rabbits and pigs.

UR - http://www.scopus.com/inward/record.url?scp=0021930664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021930664&partnerID=8YFLogxK

U2 - 10.1038/315680a0

DO - 10.1038/315680a0

M3 - Article

C2 - 3892305

AN - SCOPUS:0021930664

VL - 315

SP - 680

EP - 683

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6021

ER -