Profiles of cytokine mrna expressed by dendritic epidermal T cells in mice

Hiroyuki Matsue, Ponciano D Cruz, Paul R. Bergstresser, Akira Takashima

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The epidermis of mice contains, in addition to Langerhans cells, a second dendritic population that is Thy-1+/CD3+/ CD4-/CD8-/T-cell receptor-Vγ3/Vδ1+. These dendritic epidermal γδ T cells (DETC) are now thought to comprise one element in the family of epithelial tissue-resident γδ T cells. In the present study, DETCs were examined for their expression of mRNA for cytokines, using a reverse transcription-polymerase chain reaction. Freshly isolated Thy-1+ epidermal cells constitutively expressed mRNA for γ-interferon, but not IL-2. Within 24 h after stimulation with Con A, these cells then expressed mRNA for γ-interferon and IL-2, but not IL-4. The rapid onset of expression of mRNA for IL-2 occurred exclusively within the Thy-1+ population, and in a Con A-dependent fashion. When freshly isolated epidermal cells were first stimulated with Con A and then expanded in bulk with rIL-2 for 20-24 d, cells expressing IL-4 mRNA then emerged, upon secondary stimulation with Con A. These "short-term" DETC lines also expressed mRNA for IL-2, interferon-γ, IL-1α, IL-3, IL-6, IL-7, tumor necrosis factor α and β, and granulocyte macrophage-colony stimulating factor. Interestingly, mRNA for IL-4 and IL-6 was no longer detected in long-term (> 1 year) DETC lines 7-17 and 12-12. In addition, one line (7-17) maintained IL-3 mRNA expression, whereas another (12-12) had lost this capacity. These results emphasize the concept that, as resident cells in epidermis, DETCs exhibit several different immunorelevant activities, and the heterogeneity in cytokine mRNA profiles suggests that DETCs may divide into functional subsets.

Original languageEnglish (US)
Pages (from-to)537-542
Number of pages6
JournalJournal of Investigative Dermatology
Volume101
Issue number4
StatePublished - Oct 1993

Fingerprint

T-cells
Cytokines
T-Lymphocytes
Messenger RNA
Interleukin-2
Interleukin-4
Interferons
Interleukin-3
Epidermis
Interleukin-6
Cell Line
Interleukin-7
Langerhans Cells
Polymerase chain reaction
Transcription
Granulocyte-Macrophage Colony-Stimulating Factor
T-Cell Antigen Receptor
Interleukin-1
Population
Reverse Transcription

Keywords

  • cytokine/dendritic epidermal T cells/γδ T cells

ASJC Scopus subject areas

  • Dermatology

Cite this

Profiles of cytokine mrna expressed by dendritic epidermal T cells in mice. / Matsue, Hiroyuki; Cruz, Ponciano D; Bergstresser, Paul R.; Takashima, Akira.

In: Journal of Investigative Dermatology, Vol. 101, No. 4, 10.1993, p. 537-542.

Research output: Contribution to journalArticle

Matsue, H, Cruz, PD, Bergstresser, PR & Takashima, A 1993, 'Profiles of cytokine mrna expressed by dendritic epidermal T cells in mice', Journal of Investigative Dermatology, vol. 101, no. 4, pp. 537-542.
Matsue, Hiroyuki ; Cruz, Ponciano D ; Bergstresser, Paul R. ; Takashima, Akira. / Profiles of cytokine mrna expressed by dendritic epidermal T cells in mice. In: Journal of Investigative Dermatology. 1993 ; Vol. 101, No. 4. pp. 537-542.
@article{68711d1c77114622aa13fa28c8398f4e,
title = "Profiles of cytokine mrna expressed by dendritic epidermal T cells in mice",
abstract = "The epidermis of mice contains, in addition to Langerhans cells, a second dendritic population that is Thy-1+/CD3+/ CD4-/CD8-/T-cell receptor-Vγ3/Vδ1+. These dendritic epidermal γδ T cells (DETC) are now thought to comprise one element in the family of epithelial tissue-resident γδ T cells. In the present study, DETCs were examined for their expression of mRNA for cytokines, using a reverse transcription-polymerase chain reaction. Freshly isolated Thy-1+ epidermal cells constitutively expressed mRNA for γ-interferon, but not IL-2. Within 24 h after stimulation with Con A, these cells then expressed mRNA for γ-interferon and IL-2, but not IL-4. The rapid onset of expression of mRNA for IL-2 occurred exclusively within the Thy-1+ population, and in a Con A-dependent fashion. When freshly isolated epidermal cells were first stimulated with Con A and then expanded in bulk with rIL-2 for 20-24 d, cells expressing IL-4 mRNA then emerged, upon secondary stimulation with Con A. These {"}short-term{"} DETC lines also expressed mRNA for IL-2, interferon-γ, IL-1α, IL-3, IL-6, IL-7, tumor necrosis factor α and β, and granulocyte macrophage-colony stimulating factor. Interestingly, mRNA for IL-4 and IL-6 was no longer detected in long-term (> 1 year) DETC lines 7-17 and 12-12. In addition, one line (7-17) maintained IL-3 mRNA expression, whereas another (12-12) had lost this capacity. These results emphasize the concept that, as resident cells in epidermis, DETCs exhibit several different immunorelevant activities, and the heterogeneity in cytokine mRNA profiles suggests that DETCs may divide into functional subsets.",
keywords = "cytokine/dendritic epidermal T cells/γδ T cells",
author = "Hiroyuki Matsue and Cruz, {Ponciano D} and Bergstresser, {Paul R.} and Akira Takashima",
year = "1993",
month = "10",
language = "English (US)",
volume = "101",
pages = "537--542",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Profiles of cytokine mrna expressed by dendritic epidermal T cells in mice

AU - Matsue, Hiroyuki

AU - Cruz, Ponciano D

AU - Bergstresser, Paul R.

AU - Takashima, Akira

PY - 1993/10

Y1 - 1993/10

N2 - The epidermis of mice contains, in addition to Langerhans cells, a second dendritic population that is Thy-1+/CD3+/ CD4-/CD8-/T-cell receptor-Vγ3/Vδ1+. These dendritic epidermal γδ T cells (DETC) are now thought to comprise one element in the family of epithelial tissue-resident γδ T cells. In the present study, DETCs were examined for their expression of mRNA for cytokines, using a reverse transcription-polymerase chain reaction. Freshly isolated Thy-1+ epidermal cells constitutively expressed mRNA for γ-interferon, but not IL-2. Within 24 h after stimulation with Con A, these cells then expressed mRNA for γ-interferon and IL-2, but not IL-4. The rapid onset of expression of mRNA for IL-2 occurred exclusively within the Thy-1+ population, and in a Con A-dependent fashion. When freshly isolated epidermal cells were first stimulated with Con A and then expanded in bulk with rIL-2 for 20-24 d, cells expressing IL-4 mRNA then emerged, upon secondary stimulation with Con A. These "short-term" DETC lines also expressed mRNA for IL-2, interferon-γ, IL-1α, IL-3, IL-6, IL-7, tumor necrosis factor α and β, and granulocyte macrophage-colony stimulating factor. Interestingly, mRNA for IL-4 and IL-6 was no longer detected in long-term (> 1 year) DETC lines 7-17 and 12-12. In addition, one line (7-17) maintained IL-3 mRNA expression, whereas another (12-12) had lost this capacity. These results emphasize the concept that, as resident cells in epidermis, DETCs exhibit several different immunorelevant activities, and the heterogeneity in cytokine mRNA profiles suggests that DETCs may divide into functional subsets.

AB - The epidermis of mice contains, in addition to Langerhans cells, a second dendritic population that is Thy-1+/CD3+/ CD4-/CD8-/T-cell receptor-Vγ3/Vδ1+. These dendritic epidermal γδ T cells (DETC) are now thought to comprise one element in the family of epithelial tissue-resident γδ T cells. In the present study, DETCs were examined for their expression of mRNA for cytokines, using a reverse transcription-polymerase chain reaction. Freshly isolated Thy-1+ epidermal cells constitutively expressed mRNA for γ-interferon, but not IL-2. Within 24 h after stimulation with Con A, these cells then expressed mRNA for γ-interferon and IL-2, but not IL-4. The rapid onset of expression of mRNA for IL-2 occurred exclusively within the Thy-1+ population, and in a Con A-dependent fashion. When freshly isolated epidermal cells were first stimulated with Con A and then expanded in bulk with rIL-2 for 20-24 d, cells expressing IL-4 mRNA then emerged, upon secondary stimulation with Con A. These "short-term" DETC lines also expressed mRNA for IL-2, interferon-γ, IL-1α, IL-3, IL-6, IL-7, tumor necrosis factor α and β, and granulocyte macrophage-colony stimulating factor. Interestingly, mRNA for IL-4 and IL-6 was no longer detected in long-term (> 1 year) DETC lines 7-17 and 12-12. In addition, one line (7-17) maintained IL-3 mRNA expression, whereas another (12-12) had lost this capacity. These results emphasize the concept that, as resident cells in epidermis, DETCs exhibit several different immunorelevant activities, and the heterogeneity in cytokine mRNA profiles suggests that DETCs may divide into functional subsets.

KW - cytokine/dendritic epidermal T cells/γδ T cells

UR - http://www.scopus.com/inward/record.url?scp=0027490120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027490120&partnerID=8YFLogxK

M3 - Article

VL - 101

SP - 537

EP - 542

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 4

ER -