Profiling antibodies to class II HLA in transplant patient sera

Curtis McMurtrey, Dave Lowe, Rico Buchli, Sunil Daga, Derek Royer, Alisha Humphrey, Steven Cate, Sean Osborn, Aleksandar Mojsilovic, Rodney VanGundy, Wilfried Bardet, Andrew Duty, Danijela Mojsilovic, Kenneth Jackson, Peter Stastny, David Briggs, Daniel Zehnder, Rob Higgins, William Hildebrand

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Immunizing events including pregnancy, transfusions, and transplantation promote strong alloantibody responses to HLA. Such alloantibodies to HLA preclude organ transplantation, foster hyperacute rejection, and contribute to chronic transplant failure. Diagnostic antibody-screening assays detect alloreactive antibodies, yet key attributes including antibody concentration and isotype remain largely unexplored. The goal here was to provide a detailed profile of allogeneic antibodies to class II HLA. Methodologically, alloantibodies were purified from sensitized patient sera using an HLA-DR11 immunoaffinity column and subsequently categorized. Antibodies to DR11 were found to fix complement, exist at a median serum concentration of 2.3. μg/mL, consist of all isotypes, and isotypes IgG2, IgM, and IgE were elevated. Because multimeric isotypes can confound diagnostic determinations of antibody concentration, IgM and IgA isotypes were removed and DR11-IgG tested alone. Despite removal of multimeric isotypes, patient-to-patient antibody concentrations did not correlate with MFI values. In conclusion, allogeneic antibody responses to DR11 are comprised of all antibody isotypes at differing proportions, these combined isotypes fix complement at nominal serum concentrations, and enhancements other than the removal of IgM and IgA multimeric isotypes may be required if MFI is to be used as a means of determining anti-HLA serum antibody concentrations in diagnostic clinical assays.

Original languageEnglish (US)
Pages (from-to)261-270
Number of pages10
JournalHuman Immunology
Volume75
Issue number3
DOIs
StatePublished - Mar 2014

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Profiling antibodies to class II HLA in transplant patient sera'. Together they form a unique fingerprint.

  • Cite this

    McMurtrey, C., Lowe, D., Buchli, R., Daga, S., Royer, D., Humphrey, A., Cate, S., Osborn, S., Mojsilovic, A., VanGundy, R., Bardet, W., Duty, A., Mojsilovic, D., Jackson, K., Stastny, P., Briggs, D., Zehnder, D., Higgins, R., & Hildebrand, W. (2014). Profiling antibodies to class II HLA in transplant patient sera. Human Immunology, 75(3), 261-270. https://doi.org/10.1016/j.humimm.2013.11.015