Profiling cellular morphodynamics by spatiotemporal spectrum decomposition

Xiao Ma, Onur Dagliyan, Klaus M. Hahn, Gaudenz Danuser

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cellular morphology and associated morphodynamics are widely used for qualitative and quantitative assessments of cell state. Here we implement a framework to profile cellular morphodynamics based on an adaptive decomposition of local cell boundary motion into instantaneous frequency spectra defined by the Hilbert-Huang transform (HHT). Our approach revealed that spontaneously migrating cells with approximately homogeneous molecular makeup show remarkably consistent instantaneous frequency distributions, though they have markedly heterogeneous mobility. Distinctions in cell edge motion between these cells are captured predominantly by differences in the magnitude of the frequencies. We found that acute photo-inhibition of Vav2 guanine exchange factor, an activator of the Rho family of signaling proteins coordinating cell motility, produces significant shifts in the frequency distribution, but does not affect frequency magnitude. We therefore concluded that the frequency spectrum encodes the wiring of the molecular circuitry that regulates cell boundary movements, whereas the magnitude captures the activation level of the circuitry. We also used HHT spectra as multi-scale spatiotemporal features in statistical region merging to identify subcellular regions of distinct motion behavior. In line with our conclusion that different HHT spectra relate to different signaling regimes, we found that subcellular regions with different morphodynamics indeed exhibit distinct Rac1 activities. This algorithm thus can serve as an accurate and sensitive classifier of cellular morphodynamics to pinpoint spatial and temporal boundaries between signaling regimes.

Original languageEnglish (US)
Article numbere1006321
JournalPLoS Computational Biology
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2018

Fingerprint

morphodynamics
Profiling
decomposition
Hilbert-Huang Transform
Decomposition
Decompose
transform
degradation
Cell
Instantaneous Frequency
Frequency Spectrum
Cell Movement
cells
Electric wiring
Rho Factor
Merging
photoinhibition
Motion
motility
Classifiers

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Profiling cellular morphodynamics by spatiotemporal spectrum decomposition. / Ma, Xiao; Dagliyan, Onur; Hahn, Klaus M.; Danuser, Gaudenz.

In: PLoS Computational Biology, Vol. 14, No. 8, e1006321, 01.08.2018.

Research output: Contribution to journalArticle

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