Progenitors of dorsal commissural interneurons are defined by MATH1 expression

Amy W. Helms, Jane E. Johnson

Research output: Contribution to journalArticle

221 Citations (Scopus)

Abstract

MATH1 is a neural-specific basic helix-loop-helix transcription factor. Members of this family of transcription factors are involved in the development of specific subsets of neurons in the developing vertebrate nervous system. Here we examine the cells expressing MATH1 with respect to their proliferative state and coexpression of cell-type-specific differentiation markers. We localize the MATH1 protein to the nucleus of cells in the dorsal neural tube and the external germinal layer (EGL) of the developing cerebellum. Using double-label immunofluorescence, we demonstrate that MATH1-expressing cells span both the proliferating and the differentiating zones within the dorsal neural tube, but within the EGL of the cerebellum are restricted to the proliferating zone. The early differentiating MATH1-expressing cells in the dorsal neural tube co-express TAG-1, DCC-1 and LH2, markers of dorsal commissural interneurons. In addition, transgenic mice with lacZ under the transcriptional control of MATH1-flanking DNA sequence express β-galactosidase specifically in the developing nervous system, in a manner that mimics subsets of the MATH1-expression pattern, including the dorsal spinal neural tube. Expression of the MATH1/lacZ transgene persists in differentiated dorsal commissural interneurons. Taken together, we demonstrate MATH1 expression in a differentiating population of neuronal precursors in the dorsal neural tube that appear to give rise specifically to dorsal commissural interneurons.

Original languageEnglish (US)
Pages (from-to)919-928
Number of pages10
JournalDevelopment
Volume125
Issue number5
StatePublished - 1998

Fingerprint

Neural Tube
Cerebellum
Nervous System
Galactosidases
Basic Helix-Loop-Helix Transcription Factors
Differentiation Antigens
Cell Nucleus
Transgenes
Transgenic Mice
Fluorescent Antibody Technique
Vertebrates
Cell Differentiation
Transcription Factors
Commissural Interneurons
Neurons
Population
Proteins

Keywords

  • bHLH transcription factor
  • Cerebellum
  • MATH1
  • Mouse
  • Neural tube
  • Neuron
  • Spinal cord

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Progenitors of dorsal commissural interneurons are defined by MATH1 expression. / Helms, Amy W.; Johnson, Jane E.

In: Development, Vol. 125, No. 5, 1998, p. 919-928.

Research output: Contribution to journalArticle

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