Prognostic significance of altered p120ctn expression in bladder cancer

Brasil Silva Neto, Gjanje L. Smith, Jessica A. Mandeville, Alex J. Vanni, Chad Wotkowicz, Kimberly M. Rieger-Christ, Egbert Baumgart, Micah A. Jacobs, Michael S. Cohen, Ron Zeheb, Massimo Loda, John A. Libertino, Ian C. Summerhayes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

OBJECTIVE: To identify the frequency of change in the expression and localization of p120ctn in bladder tumours and its association with clinical outcomes, and to investigate the potential role of p120ctn in the migratory and invasive behaviour of bladder carcinoma cells. MATERIALS AND METHODS: In all, 425 superficial tumour specimens (Ta, Tis and T1) and 305 invasive (T2-T4) tumour specimens from 534 patients were assembled in 10 tissue microarrays. P120ctn immunostaining was scored for intensity and cellular localization and correlated with clinical variables and survival analysis. Knockdown of p120ctn was achieved using small-interference RNA (siRNA) followed by the assessment of migration and invasion behaviour in standard in vitro assays. RESULTS: The expression levels of p120 catenin inversely correlated with pathological tumour stage (P < 0.001), histological grade (P < 0.001), presence of lymphovascular invasion (P = 0.02) but not lymph node (LN) involvement (P = 0.17). Non-membranous localization of p120 ctn correlated with stage (P < 0.001), grade (P < 0.001), lymphovascular invasion (P = 0.04) and LN-positive disease (P = 0.02). A low expression level of p120ctn was linked to a poor outcome in cancer-specific survival analysis. Knockdown of p120ctn using siRNA resulted in a significant reduction in the migration and invasive potential of bladder carcinoma cells. CONCLUSIONS: Our findings suggest that p120 ctn acts as a prognostic factor in bladder tumours and has a primary role to play in the migratory and invasive behaviour of bladder carcinoma cells.

Original languageEnglish (US)
Pages (from-to)746-752
Number of pages7
JournalBJU International
Volume101
Issue number6
DOIs
StatePublished - Mar 2008

Fingerprint

Urinary Bladder Neoplasms
Urinary Bladder
Survival Analysis
RNA Interference
Carcinoma
Neoplasms
Lymph Nodes
delta catenin

Keywords

  • Bladder cancer
  • p120
  • Tissue microarrays

ASJC Scopus subject areas

  • Urology

Cite this

Neto, B. S., Smith, G. L., Mandeville, J. A., Vanni, A. J., Wotkowicz, C., Rieger-Christ, K. M., ... Summerhayes, I. C. (2008). Prognostic significance of altered p120ctn expression in bladder cancer. BJU International, 101(6), 746-752. https://doi.org/10.1111/j.1464-410X.2007.07264.x

Prognostic significance of altered p120ctn expression in bladder cancer. / Neto, Brasil Silva; Smith, Gjanje L.; Mandeville, Jessica A.; Vanni, Alex J.; Wotkowicz, Chad; Rieger-Christ, Kimberly M.; Baumgart, Egbert; Jacobs, Micah A.; Cohen, Michael S.; Zeheb, Ron; Loda, Massimo; Libertino, John A.; Summerhayes, Ian C.

In: BJU International, Vol. 101, No. 6, 03.2008, p. 746-752.

Research output: Contribution to journalArticle

Neto, BS, Smith, GL, Mandeville, JA, Vanni, AJ, Wotkowicz, C, Rieger-Christ, KM, Baumgart, E, Jacobs, MA, Cohen, MS, Zeheb, R, Loda, M, Libertino, JA & Summerhayes, IC 2008, 'Prognostic significance of altered p120ctn expression in bladder cancer', BJU International, vol. 101, no. 6, pp. 746-752. https://doi.org/10.1111/j.1464-410X.2007.07264.x
Neto BS, Smith GL, Mandeville JA, Vanni AJ, Wotkowicz C, Rieger-Christ KM et al. Prognostic significance of altered p120ctn expression in bladder cancer. BJU International. 2008 Mar;101(6):746-752. https://doi.org/10.1111/j.1464-410X.2007.07264.x
Neto, Brasil Silva ; Smith, Gjanje L. ; Mandeville, Jessica A. ; Vanni, Alex J. ; Wotkowicz, Chad ; Rieger-Christ, Kimberly M. ; Baumgart, Egbert ; Jacobs, Micah A. ; Cohen, Michael S. ; Zeheb, Ron ; Loda, Massimo ; Libertino, John A. ; Summerhayes, Ian C. / Prognostic significance of altered p120ctn expression in bladder cancer. In: BJU International. 2008 ; Vol. 101, No. 6. pp. 746-752.
@article{7c8906a447b2490d80ccc9df6259a712,
title = "Prognostic significance of altered p120ctn expression in bladder cancer",
abstract = "OBJECTIVE: To identify the frequency of change in the expression and localization of p120ctn in bladder tumours and its association with clinical outcomes, and to investigate the potential role of p120ctn in the migratory and invasive behaviour of bladder carcinoma cells. MATERIALS AND METHODS: In all, 425 superficial tumour specimens (Ta, Tis and T1) and 305 invasive (T2-T4) tumour specimens from 534 patients were assembled in 10 tissue microarrays. P120ctn immunostaining was scored for intensity and cellular localization and correlated with clinical variables and survival analysis. Knockdown of p120ctn was achieved using small-interference RNA (siRNA) followed by the assessment of migration and invasion behaviour in standard in vitro assays. RESULTS: The expression levels of p120 catenin inversely correlated with pathological tumour stage (P < 0.001), histological grade (P < 0.001), presence of lymphovascular invasion (P = 0.02) but not lymph node (LN) involvement (P = 0.17). Non-membranous localization of p120 ctn correlated with stage (P < 0.001), grade (P < 0.001), lymphovascular invasion (P = 0.04) and LN-positive disease (P = 0.02). A low expression level of p120ctn was linked to a poor outcome in cancer-specific survival analysis. Knockdown of p120ctn using siRNA resulted in a significant reduction in the migration and invasive potential of bladder carcinoma cells. CONCLUSIONS: Our findings suggest that p120 ctn acts as a prognostic factor in bladder tumours and has a primary role to play in the migratory and invasive behaviour of bladder carcinoma cells.",
keywords = "Bladder cancer, p120, Tissue microarrays",
author = "Neto, {Brasil Silva} and Smith, {Gjanje L.} and Mandeville, {Jessica A.} and Vanni, {Alex J.} and Chad Wotkowicz and Rieger-Christ, {Kimberly M.} and Egbert Baumgart and Jacobs, {Micah A.} and Cohen, {Michael S.} and Ron Zeheb and Massimo Loda and Libertino, {John A.} and Summerhayes, {Ian C.}",
year = "2008",
month = "3",
doi = "10.1111/j.1464-410X.2007.07264.x",
language = "English (US)",
volume = "101",
pages = "746--752",
journal = "BJU International",
issn = "1464-4096",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Prognostic significance of altered p120ctn expression in bladder cancer

AU - Neto, Brasil Silva

AU - Smith, Gjanje L.

AU - Mandeville, Jessica A.

AU - Vanni, Alex J.

AU - Wotkowicz, Chad

AU - Rieger-Christ, Kimberly M.

AU - Baumgart, Egbert

AU - Jacobs, Micah A.

AU - Cohen, Michael S.

AU - Zeheb, Ron

AU - Loda, Massimo

AU - Libertino, John A.

AU - Summerhayes, Ian C.

PY - 2008/3

Y1 - 2008/3

N2 - OBJECTIVE: To identify the frequency of change in the expression and localization of p120ctn in bladder tumours and its association with clinical outcomes, and to investigate the potential role of p120ctn in the migratory and invasive behaviour of bladder carcinoma cells. MATERIALS AND METHODS: In all, 425 superficial tumour specimens (Ta, Tis and T1) and 305 invasive (T2-T4) tumour specimens from 534 patients were assembled in 10 tissue microarrays. P120ctn immunostaining was scored for intensity and cellular localization and correlated with clinical variables and survival analysis. Knockdown of p120ctn was achieved using small-interference RNA (siRNA) followed by the assessment of migration and invasion behaviour in standard in vitro assays. RESULTS: The expression levels of p120 catenin inversely correlated with pathological tumour stage (P < 0.001), histological grade (P < 0.001), presence of lymphovascular invasion (P = 0.02) but not lymph node (LN) involvement (P = 0.17). Non-membranous localization of p120 ctn correlated with stage (P < 0.001), grade (P < 0.001), lymphovascular invasion (P = 0.04) and LN-positive disease (P = 0.02). A low expression level of p120ctn was linked to a poor outcome in cancer-specific survival analysis. Knockdown of p120ctn using siRNA resulted in a significant reduction in the migration and invasive potential of bladder carcinoma cells. CONCLUSIONS: Our findings suggest that p120 ctn acts as a prognostic factor in bladder tumours and has a primary role to play in the migratory and invasive behaviour of bladder carcinoma cells.

AB - OBJECTIVE: To identify the frequency of change in the expression and localization of p120ctn in bladder tumours and its association with clinical outcomes, and to investigate the potential role of p120ctn in the migratory and invasive behaviour of bladder carcinoma cells. MATERIALS AND METHODS: In all, 425 superficial tumour specimens (Ta, Tis and T1) and 305 invasive (T2-T4) tumour specimens from 534 patients were assembled in 10 tissue microarrays. P120ctn immunostaining was scored for intensity and cellular localization and correlated with clinical variables and survival analysis. Knockdown of p120ctn was achieved using small-interference RNA (siRNA) followed by the assessment of migration and invasion behaviour in standard in vitro assays. RESULTS: The expression levels of p120 catenin inversely correlated with pathological tumour stage (P < 0.001), histological grade (P < 0.001), presence of lymphovascular invasion (P = 0.02) but not lymph node (LN) involvement (P = 0.17). Non-membranous localization of p120 ctn correlated with stage (P < 0.001), grade (P < 0.001), lymphovascular invasion (P = 0.04) and LN-positive disease (P = 0.02). A low expression level of p120ctn was linked to a poor outcome in cancer-specific survival analysis. Knockdown of p120ctn using siRNA resulted in a significant reduction in the migration and invasive potential of bladder carcinoma cells. CONCLUSIONS: Our findings suggest that p120 ctn acts as a prognostic factor in bladder tumours and has a primary role to play in the migratory and invasive behaviour of bladder carcinoma cells.

KW - Bladder cancer

KW - p120

KW - Tissue microarrays

UR - http://www.scopus.com/inward/record.url?scp=39449113388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39449113388&partnerID=8YFLogxK

U2 - 10.1111/j.1464-410X.2007.07264.x

DO - 10.1111/j.1464-410X.2007.07264.x

M3 - Article

C2 - 17922855

AN - SCOPUS:39449113388

VL - 101

SP - 746

EP - 752

JO - BJU International

JF - BJU International

SN - 1464-4096

IS - 6

ER -