Program specificity for ptf1a in pancreas versus neural tube development correlates with distinct collaborating cofactors and chromatin accessibility

David M. Meredith, Mark D. Borromeo, Tye G. Deering, Bradford H. Casey, Trisha K. Savage, Paul R. Mayer, Chinh Hoang, Kuang Chi Tung, Manonmani Kumar, Chengcheng Shen, Galvin H. Swift, Raymond J. MacDonald, Jane E. Johnsona

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The lineage-specific basic helix-loop-helix transcription factor Ptf1a is a critical driver for development of both the pancreas and nervous system. How one transcription factor controls diverse programs of gene expression is a fundamental question in developmental biology. To uncover molecular strategies for the program-specific functions of Ptf1a, we identified bound genomic regions in vivo during development of both tissues. Most regions bound by Ptf1a are specific to each tissue, lie near genes needed for proper formation of each tissue, and coincide with regions of open chromatin. The specificity of Ptf1a binding is encoded in the DNA surrounding the Ptf1a-bound sites, because these regions are sufficient to direct tissue-restricted reporter expression in transgenic mice. Fox and Sox factors were identified as potential lineage-specific modifiers of Ptf1a binding, since binding motifs for these factors are enriched in Ptf1a-bound regions in pancreas and neural tube, respectively. Of the Fox factors expressed during pancreatic development, Foxa2 plays a major role. Indeed, Ptf1a and Foxa2 colocalize in embryonic pancreatic chromatin and can act synergistically in cell transfection assays. Together, these findings indicate that lineage-specific chromatin landscapes likely constrain the DNA binding of Ptf1a, and they identify Fox and Sox gene families as part of this process

Original languageEnglish (US)
Pages (from-to)3166-3179
Number of pages14
JournalMolecular and Cellular Biology
Volume33
Issue number16
DOIs
StatePublished - 2013

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Neural Tube
Chromatin
Pancreas
Basic Helix-Loop-Helix Transcription Factors
Developmental Biology
DNA
Nervous System
Transgenic Mice
Genes
Transfection
Transcription Factors
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Meredith, D. M., Borromeo, M. D., Deering, T. G., Casey, B. H., Savage, T. K., Mayer, P. R., ... Johnsona, J. E. (2013). Program specificity for ptf1a in pancreas versus neural tube development correlates with distinct collaborating cofactors and chromatin accessibility. Molecular and Cellular Biology, 33(16), 3166-3179. https://doi.org/10.1128/MCB.00364-13

Program specificity for ptf1a in pancreas versus neural tube development correlates with distinct collaborating cofactors and chromatin accessibility. / Meredith, David M.; Borromeo, Mark D.; Deering, Tye G.; Casey, Bradford H.; Savage, Trisha K.; Mayer, Paul R.; Hoang, Chinh; Tung, Kuang Chi; Kumar, Manonmani; Shen, Chengcheng; Swift, Galvin H.; MacDonald, Raymond J.; Johnsona, Jane E.

In: Molecular and Cellular Biology, Vol. 33, No. 16, 2013, p. 3166-3179.

Research output: Contribution to journalArticle

Meredith, DM, Borromeo, MD, Deering, TG, Casey, BH, Savage, TK, Mayer, PR, Hoang, C, Tung, KC, Kumar, M, Shen, C, Swift, GH, MacDonald, RJ & Johnsona, JE 2013, 'Program specificity for ptf1a in pancreas versus neural tube development correlates with distinct collaborating cofactors and chromatin accessibility', Molecular and Cellular Biology, vol. 33, no. 16, pp. 3166-3179. https://doi.org/10.1128/MCB.00364-13
Meredith, David M. ; Borromeo, Mark D. ; Deering, Tye G. ; Casey, Bradford H. ; Savage, Trisha K. ; Mayer, Paul R. ; Hoang, Chinh ; Tung, Kuang Chi ; Kumar, Manonmani ; Shen, Chengcheng ; Swift, Galvin H. ; MacDonald, Raymond J. ; Johnsona, Jane E. / Program specificity for ptf1a in pancreas versus neural tube development correlates with distinct collaborating cofactors and chromatin accessibility. In: Molecular and Cellular Biology. 2013 ; Vol. 33, No. 16. pp. 3166-3179.
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