Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori in patients with different clinical presentations

Per Syrak Hansen, Mae F. Go, Kim Varming, Leif P. Andersen, Robert M. Genta, David Y. Graham, Henrik Nielsen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Chronic Helicobacter pylori infection is associated with mucosal inflammation. The aim of the present study was to assess human neutrophil and monocyte activation by H. pylori strains obtained from patients with different clinical presentations. Bacterial sonicates from 12 strains were used to stimulate phagocyte upregulation of CD11b/CD18 adherence molecules assessed by fluorescence flow cytometry and oxidative burst responses assessed by chemiluminescence. A dose-dependent activation of CD11b/CD18 adherence molecules was observed with all strains on both neutrophils and monocytes. The activities were similar for strains from patients with duodenal ulceration and for strains from asymptomatic volunteers irrespective of histopathologic grades of the biopsy specimens from the antral mucosa. The neutrophil chemiluminescence response correlated with histopathologic severity. We conclude that upregulation of neutrophil and monocyte adherence molecules by H. pylori sonicates is not associated with clinical presentation of the infection.

Original languageEnglish (US)
Pages (from-to)3171-3174
Number of pages4
JournalInfection and Immunity
Volume67
Issue number6
StatePublished - Jun 1999

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Neutrophil Activation
Helicobacter pylori
Monocytes
Neutrophils
Luminescence
Up-Regulation
Respiratory Burst
Helicobacter Infections
Phagocytes
Volunteers
Flow Cytometry
Mucous Membrane
Fluorescence
Inflammation
Biopsy
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Hansen, P. S., Go, M. F., Varming, K., Andersen, L. P., Genta, R. M., Graham, D. Y., & Nielsen, H. (1999). Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori in patients with different clinical presentations. Infection and Immunity, 67(6), 3171-3174.

Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori in patients with different clinical presentations. / Hansen, Per Syrak; Go, Mae F.; Varming, Kim; Andersen, Leif P.; Genta, Robert M.; Graham, David Y.; Nielsen, Henrik.

In: Infection and Immunity, Vol. 67, No. 6, 06.1999, p. 3171-3174.

Research output: Contribution to journalArticle

Hansen, PS, Go, MF, Varming, K, Andersen, LP, Genta, RM, Graham, DY & Nielsen, H 1999, 'Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori in patients with different clinical presentations', Infection and Immunity, vol. 67, no. 6, pp. 3171-3174.
Hansen, Per Syrak ; Go, Mae F. ; Varming, Kim ; Andersen, Leif P. ; Genta, Robert M. ; Graham, David Y. ; Nielsen, Henrik. / Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori in patients with different clinical presentations. In: Infection and Immunity. 1999 ; Vol. 67, No. 6. pp. 3171-3174.
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