Proinflammatory fecal mRNA and childhood bacterial enteric infections

William E. Bennett, Rosbel González-Rivera, Bao N. Puente, Nurmohammad Shaikh, Harold J. Stevens, Jody C. Mooney, Eileen J. Klein, Donna M. Denno, Andrew Draghi, Francisco A. Sylvester, Phillip I. Tarr

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Results: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalGut Microbes
Volume1
Issue number4
DOIs
StatePublished - 2010

Keywords

  • Campylobacter
  • E. coli O157:H7
  • Enzyme immunoassay
  • Infectious colitis
  • Salmonella
  • Shigella
  • Stool
  • mRNA

ASJC Scopus subject areas

  • Microbiology
  • Gastroenterology
  • Microbiology (medical)
  • Infectious Diseases

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