TY - JOUR
T1 - Prolactin stimulates proliferation of cultured human keratinocytes
AU - Girolomoni, Giampiero
AU - Theodore Phillips, J.
AU - Bergstresser, Paul R.
N1 - Funding Information:
We thank Dr. Howard Green Jorgmerously providing the 3T3-J2 cel/line, Ms. Kim Meyers Jor valuable technical assistance, andMrs. BettyJ anes Jor her expert secretarial assistance. This work was supported by National Institutes oj Health Grants R01-AR35068 (PRB), R01-AR40042 (PRB), and R29-DK39526 (]TP), and by a Research Grant from Mary Kay Cosmetics, bIC., Dal/as, TX.
PY - 1993/9
Y1 - 1993/9
N2 - The effect of the pituitary hormone prolactin on in vitro proliferation of human keratinocytes has been studied. Cell proliferation was determined by [3H] thymidine incorporation and cell enumeration in culture. Physiologic concentrations of prolactin markedly stimulated proliferation of newborn foreskin keratinocytes in serum-free medium. In addition, it was able to replace almost completely the growth-promoting effects of bovine pituitary extract, a commonly added supplement for keratinocyte culture. This activity was also evident in the absence of epidermal growth factor, but required the presence of insulin. Radioligand binding studies confirmed the expression of specific prolactin binding sites (Kd 8.9 nM; 1350 sites per cell) on freshly procured keratinocyte membranes. These results extend its hormonal influences to include regulation of in vitro proliferation of human keratinocytes, and suggest the possibility of a completely defined growth medium for keratinocytes.
AB - The effect of the pituitary hormone prolactin on in vitro proliferation of human keratinocytes has been studied. Cell proliferation was determined by [3H] thymidine incorporation and cell enumeration in culture. Physiologic concentrations of prolactin markedly stimulated proliferation of newborn foreskin keratinocytes in serum-free medium. In addition, it was able to replace almost completely the growth-promoting effects of bovine pituitary extract, a commonly added supplement for keratinocyte culture. This activity was also evident in the absence of epidermal growth factor, but required the presence of insulin. Radioligand binding studies confirmed the expression of specific prolactin binding sites (Kd 8.9 nM; 1350 sites per cell) on freshly procured keratinocyte membranes. These results extend its hormonal influences to include regulation of in vitro proliferation of human keratinocytes, and suggest the possibility of a completely defined growth medium for keratinocytes.
KW - epidermis/pituitary hormones/serum-free culture/receptors
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U2 - 10.1111/1523-1747.ep12365203
DO - 10.1111/1523-1747.ep12365203
M3 - Article
C2 - 8370964
AN - SCOPUS:0027295353
SN - 0022-202X
VL - 101
SP - 275
EP - 279
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 3
ER -