Proliferation and metabolic significance of peroxisomes in Candida boidinii during growth on d-alanine or oleic acid as the sole carbon source

G. J. Sulter, H. R. Waterham, J. M. Goodman, M. Veenhuis

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We have studied the induction of peroxisomes in the methylotrophic yeast Candida boidinii by d-alanine and oleic acid. The organism was able to utilize each of these compounds as the sole carbon source and grew with growth rates of μ=0.20 h-1 (on d-alanine) or μ=0.43 h-1 (on oleic acid). Growth was associated with the development of many peroxisomes in the cells. On d-alanine a cluster of tightly interwoven organelles was observed which made up 6.3% of the cytoplasmic volume and were characterized by the presence of d-amino acid oxidase and catalase. On oleic acid rounded to elongated peroxisomes were dominant which were scattered throughout the cytoplasm. These organelles contained increased levels of β-oxidation enzymes; their relative volume fraction amounted 12.8% of the cytoplasmic volume.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalArchives of Microbiology
Volume153
Issue number5
DOIs
StatePublished - Apr 1990

Keywords

  • Candida boidinii
  • Peroxisomes
  • Yeast
  • d-Amino acid oxidase
  • β-Oxidation

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Proliferation and metabolic significance of peroxisomes in Candida boidinii during growth on d-alanine or oleic acid as the sole carbon source'. Together they form a unique fingerprint.

  • Cite this