Abstract
We have studied the induction of peroxisomes in the methylotrophic yeast Candida boidinii by d-alanine and oleic acid. The organism was able to utilize each of these compounds as the sole carbon source and grew with growth rates of μ=0.20 h-1 (on d-alanine) or μ=0.43 h-1 (on oleic acid). Growth was associated with the development of many peroxisomes in the cells. On d-alanine a cluster of tightly interwoven organelles was observed which made up 6.3% of the cytoplasmic volume and were characterized by the presence of d-amino acid oxidase and catalase. On oleic acid rounded to elongated peroxisomes were dominant which were scattered throughout the cytoplasm. These organelles contained increased levels of β-oxidation enzymes; their relative volume fraction amounted 12.8% of the cytoplasmic volume.
Original language | English (US) |
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Pages (from-to) | 485-489 |
Number of pages | 5 |
Journal | Archives of Microbiology |
Volume | 153 |
Issue number | 5 |
DOIs | |
State | Published - Apr 1990 |
Keywords
- Candida boidinii
- Peroxisomes
- Yeast
- d-Amino acid oxidase
- β-Oxidation
ASJC Scopus subject areas
- Microbiology
- Biochemistry
- Molecular Biology
- Genetics