Proliferation and migration of tumor cells in tapered channels

Yuan Wan, Deepika Tamuly, Peter B. Allen, Young Tae Kim, Robert Bachoo, Andrew D. Ellington, Samir M. Iqbal

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Tumor cells depict two deviant tendencies; over-proliferation and vigorous migration. A tapered channel device is designed and fabricated for in vitro studies. We report inhibited proliferation and migration of human glioblastoma (hGBM) cells when exposed to an aptamer that is known to bind epidermal growth factor receptors (EGFR). The device is integrated with controlled ambient and microscope for providing real-time and quantitative characterization of the tumor cell behavior. The results show that hGBM cells loose proliferation and motility when exposed to the anti-EGFR aptamer. The aptamer directly inhibits and blocks EGF-induced EGFR phosphorylation. This also reduces the ability of cells to remodel their internal structure for invasion through narrow constrictions. This provides a framework for possible studies on efficacy of other inhibiting molecules.

Original languageEnglish (US)
Pages (from-to)635-643
Number of pages9
JournalBiomedical Microdevices
Volume15
Issue number4
DOIs
StatePublished - Aug 1 2013

Keywords

  • Aggressive migration
  • Inhibition of cancer
  • Microfluidics
  • Tapered channels

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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  • Cite this

    Wan, Y., Tamuly, D., Allen, P. B., Kim, Y. T., Bachoo, R., Ellington, A. D., & Iqbal, S. M. (2013). Proliferation and migration of tumor cells in tapered channels. Biomedical Microdevices, 15(4), 635-643. https://doi.org/10.1007/s10544-012-9721-0