Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung.

P. W. Shaul, A. J. North, T. S. Brannon, K. Ujiie, L. B. Wells, P. A. Nisen, C. J. Lowenstein, S. H. Snyder, R. A. Star

Research output: Contribution to journalArticle

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Abstract

Prolonged hypoxia in the adult rat causes a decline in endothelium-derived nitric oxide (NO) production in the pulmonary circulation. To evaluate whether this is related to a decrease in endothelial NO synthase (NOS-III) expression, we determined the effects of hypobaric hypoxia (7 or 21 days) on NOS-III gene expression in adult rat lung. Neuronal NOS (NOS-I) expression was also examined; NOS-I has been immunohistochemically localized to rat bronchiolar epithelium. NOS-III and NOS-I mRNA abundance were assessed in reverse transcription-polymerase chain reaction assays and the proteins were evaluated by immunoblot analysis. After 7 and 21 days of hypoxia, there were increases in the steady-state levels of both NOS-III and NOS-I mRNA, rising 2.7- to 3.0-fold and 2.5- to 2.8-fold, respectively. These findings were confirmed by Northern analyses. In parallel, NOS-III and NOS-I protein abundance were also increased with hypoxia by 3.0- to 3.5-fold and 2.4- to 3.0-fold, respectively. NOS activity detected by [3H]arginine to [3H]citrulline conversion rose 109%. Thus, prolonged in vivo hypoxia causes enhancement of NOS-III and NOS-I gene expression in adult rat lung, indicating that the pulmonary expression of these genes is modulated in vivo. The increase in NOS-III expression does not explain the declines in pulmonary endothelial NO production previously observed following prolonged hypoxia in this model. Alternatively, the fall in NO production may be related to diminished NOS co-factor availability.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume13
Issue number2
StatePublished - Aug 1995

Fingerprint

Nitric Oxide Synthase Type I
Gene expression
Rats
Gene Expression
Lung
Nitric Oxide
Citrulline
Messenger RNA
Nitric Oxide Synthase Type III
Polymerase chain reaction
Transcription
Arginine
Assays
Proteins
Pulmonary Circulation
Genes
Availability
Reverse Transcription
Hypoxia
Epithelium

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung. / Shaul, P. W.; North, A. J.; Brannon, T. S.; Ujiie, K.; Wells, L. B.; Nisen, P. A.; Lowenstein, C. J.; Snyder, S. H.; Star, R. A.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 13, No. 2, 08.1995, p. 167-174.

Research output: Contribution to journalArticle

Shaul, P. W. ; North, A. J. ; Brannon, T. S. ; Ujiie, K. ; Wells, L. B. ; Nisen, P. A. ; Lowenstein, C. J. ; Snyder, S. H. ; Star, R. A. / Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung. In: American Journal of Respiratory Cell and Molecular Biology. 1995 ; Vol. 13, No. 2. pp. 167-174.
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