Prolonged proliferation of primary chicken embryo fibroblasts transfected with cDNAs from the BamHI-H gene family of Marek's disease virus

F. Peng, J. Donovan, S. Specter, A. Tanaka, M. Nonoyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Oncogenic virus-specific 1.69 kb and 1.5 kb cDNAs, derived from the tumorigenicity-associated BamHI-H gene family of Marek's disease virus (MDV), were cloned into the eukaryotic expression vector (PRC/CMV) and designated as PRC/CMV-1.69 and PRC/CMV-1.5, respectively. Chicken embryo fibroblasts (CEFs) transfected with PRC/CMV-1.69 or PRC/CMV-1.5 plasmid DNA showed reduced serum-dependence for growth compared with PRC/CMV vector-transfected CEFs. Also, PRC/CMV-1.69 or PRC/CMV-1.5 DNA transfected CEFs proliferated for a longer period of time (20-22 passages) compared with the CEFs transfected with PRC/CMV plasmid DNA (10 passages). Reduced serum-dependence for growth and prolonged proliferation of CEFs after in vitro transfection with PRC/CMV- 1.69 or PRC/CMV-1.5 indicated that these two cDNAs stimulate cell growth. These data indicated that one of the functions of the BamHI-H gene family of MDV is cell-growth control, which may play an important role in the establishment and maintenance of uncontrolled growth of tumor cells induced by MDV.

Original languageEnglish (US)
Pages (from-to)587-591
Number of pages5
JournalInternational Journal of Oncology
Volume3
Issue number4
StatePublished - 1993

Fingerprint

Marek Disease
Chickens
Embryonic Structures
Complementary DNA
Fibroblasts
Viruses
Growth
Genes
DNA
Plasmids
Oncogenic Viruses
Serum
Transfection
Maintenance
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Prolonged proliferation of primary chicken embryo fibroblasts transfected with cDNAs from the BamHI-H gene family of Marek's disease virus. / Peng, F.; Donovan, J.; Specter, S.; Tanaka, A.; Nonoyama, M.

In: International Journal of Oncology, Vol. 3, No. 4, 1993, p. 587-591.

Research output: Contribution to journalArticle

@article{00d86035ec4c4abbbe921ed464eac134,
title = "Prolonged proliferation of primary chicken embryo fibroblasts transfected with cDNAs from the BamHI-H gene family of Marek's disease virus",
abstract = "Oncogenic virus-specific 1.69 kb and 1.5 kb cDNAs, derived from the tumorigenicity-associated BamHI-H gene family of Marek's disease virus (MDV), were cloned into the eukaryotic expression vector (PRC/CMV) and designated as PRC/CMV-1.69 and PRC/CMV-1.5, respectively. Chicken embryo fibroblasts (CEFs) transfected with PRC/CMV-1.69 or PRC/CMV-1.5 plasmid DNA showed reduced serum-dependence for growth compared with PRC/CMV vector-transfected CEFs. Also, PRC/CMV-1.69 or PRC/CMV-1.5 DNA transfected CEFs proliferated for a longer period of time (20-22 passages) compared with the CEFs transfected with PRC/CMV plasmid DNA (10 passages). Reduced serum-dependence for growth and prolonged proliferation of CEFs after in vitro transfection with PRC/CMV- 1.69 or PRC/CMV-1.5 indicated that these two cDNAs stimulate cell growth. These data indicated that one of the functions of the BamHI-H gene family of MDV is cell-growth control, which may play an important role in the establishment and maintenance of uncontrolled growth of tumor cells induced by MDV.",
author = "F. Peng and J. Donovan and S. Specter and A. Tanaka and M. Nonoyama",
year = "1993",
language = "English (US)",
volume = "3",
pages = "587--591",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "4",

}

TY - JOUR

T1 - Prolonged proliferation of primary chicken embryo fibroblasts transfected with cDNAs from the BamHI-H gene family of Marek's disease virus

AU - Peng, F.

AU - Donovan, J.

AU - Specter, S.

AU - Tanaka, A.

AU - Nonoyama, M.

PY - 1993

Y1 - 1993

N2 - Oncogenic virus-specific 1.69 kb and 1.5 kb cDNAs, derived from the tumorigenicity-associated BamHI-H gene family of Marek's disease virus (MDV), were cloned into the eukaryotic expression vector (PRC/CMV) and designated as PRC/CMV-1.69 and PRC/CMV-1.5, respectively. Chicken embryo fibroblasts (CEFs) transfected with PRC/CMV-1.69 or PRC/CMV-1.5 plasmid DNA showed reduced serum-dependence for growth compared with PRC/CMV vector-transfected CEFs. Also, PRC/CMV-1.69 or PRC/CMV-1.5 DNA transfected CEFs proliferated for a longer period of time (20-22 passages) compared with the CEFs transfected with PRC/CMV plasmid DNA (10 passages). Reduced serum-dependence for growth and prolonged proliferation of CEFs after in vitro transfection with PRC/CMV- 1.69 or PRC/CMV-1.5 indicated that these two cDNAs stimulate cell growth. These data indicated that one of the functions of the BamHI-H gene family of MDV is cell-growth control, which may play an important role in the establishment and maintenance of uncontrolled growth of tumor cells induced by MDV.

AB - Oncogenic virus-specific 1.69 kb and 1.5 kb cDNAs, derived from the tumorigenicity-associated BamHI-H gene family of Marek's disease virus (MDV), were cloned into the eukaryotic expression vector (PRC/CMV) and designated as PRC/CMV-1.69 and PRC/CMV-1.5, respectively. Chicken embryo fibroblasts (CEFs) transfected with PRC/CMV-1.69 or PRC/CMV-1.5 plasmid DNA showed reduced serum-dependence for growth compared with PRC/CMV vector-transfected CEFs. Also, PRC/CMV-1.69 or PRC/CMV-1.5 DNA transfected CEFs proliferated for a longer period of time (20-22 passages) compared with the CEFs transfected with PRC/CMV plasmid DNA (10 passages). Reduced serum-dependence for growth and prolonged proliferation of CEFs after in vitro transfection with PRC/CMV- 1.69 or PRC/CMV-1.5 indicated that these two cDNAs stimulate cell growth. These data indicated that one of the functions of the BamHI-H gene family of MDV is cell-growth control, which may play an important role in the establishment and maintenance of uncontrolled growth of tumor cells induced by MDV.

UR - http://www.scopus.com/inward/record.url?scp=0027368537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027368537&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0027368537

VL - 3

SP - 587

EP - 591

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 4

ER -