Promigratory and procontractile growth factor environments differentially regulate cell morphogenesis

Sangmyung Rhee, Chin Han Ho, Frederick Grinnell

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Three-dimensional (3D) cell-matrix cultures provide a useful model to analyze and dissect the structural, functional, and mechanical aspects of cell-matrix interactions and motile behavior important for cell and tissue morphogenesis. In the current studies we tested the effects of serum and physiological growth factors on the morphogenetic behavior of human fibroblasts cultured on the surfaces of 3D collagen matrices. Fibroblasts in medium containing serum contracted into clusters, whereas cells in medium containing platelet-derived growth factor (PDGF) were observed to migrate as individuals. The clustering activity of serum appeared to depend on lysophosphatidic acid, required cell contraction based on inhibition by blocking Rho kinase or myosin II, and was reversed upon switching to PDGF. Oncogenic Ras transformed human fibroblasts did not exhibit serum-stimulated cell clustering. Our findings emphasize the importance of cell-specific promigratory and procontractile growth factor environments in the differential regulation of cell motile function and cell morphogenesis.

Original languageEnglish (US)
Pages (from-to)232-244
Number of pages13
JournalExperimental Cell Research
Volume316
Issue number2
DOIs
StatePublished - Jan 15 2010

Fingerprint

Morphogenesis
Intercellular Signaling Peptides and Proteins
Fibroblasts
Platelet-Derived Growth Factor
Serum
Cluster Analysis
Myosin Type II
rho-Associated Kinases
Cell Communication
Collagen
Cell Culture Techniques

Keywords

  • 3D collagen matrix
  • Cell contraction
  • Cell motility
  • Lysophosphatidic acid
  • Platelet-derived growth factor

ASJC Scopus subject areas

  • Cell Biology

Cite this

Promigratory and procontractile growth factor environments differentially regulate cell morphogenesis. / Rhee, Sangmyung; Ho, Chin Han; Grinnell, Frederick.

In: Experimental Cell Research, Vol. 316, No. 2, 15.01.2010, p. 232-244.

Research output: Contribution to journalArticle

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