TY - JOUR
T1 - Prominent sex steroid metabolism in human lymphocytes
AU - Zhou, Zhifeng
AU - Shackleton, Cedric H L
AU - Pahwa, Savita
AU - White, Perrin C.
AU - Speiser, Phyllis W.
N1 - Funding Information:
We thank Dr So Than for assistance in obtaining and separating lymphocytes and Drs Thomas Degnan and Robert Pergolizzi and staff for use of NSUH Core Laboratory Facilities. This work was supported in part by The Hausman Fund of the NSUH Department of Pediatrics (PWS), The Genentech Foundation Grant #95-199 (PWS), NIH RO1 DK 37867 (PCW).
PY - 1998/3/16
Y1 - 1998/3/16
N2 - Steroid metabolism was investigated in cultured human B-lymphoblastoid cells (B-LCL), and peripheral blood T and B cells. Gene expression was examined by reverse-transcription polymerase chain reaction amplification (RT-PCR). Appropriate sized transcripts were detected in both cultured and fresh peripheral lymphocytes for CYP11A, CYP17, HSD11L (11β-hydroxysteroid dehydrogenase I), HSD17B1 (17β-hydroxysteroid dehydrogenase type I) and SRD5A1 (5α-reductase I). B-LCL, but not T and B cells, expressed CYP11B. There was minimal expression of HSD3B1 and HSD3B2 (3β-hydroxysteroid dehydrogenase I and II) in B-LCL and T cells. Transcripts for CYP19 and HSD11K were not detected. Corresponding enzymatic activity was detectable only for 17-hydroxysteroid dehydrogenase and 5α-reductase, respectively producing testosterone and 5α-dihydrotestosterone. Steroid identities were confirmed by gas chromatography/mass spectrometry (GC/MS). One metabolite thought to be deoxycorticosterone was identified by GC/MS as 6α-hydroxypregnanolone. It was concluded that sex hormone metabolism, including androgen synthesis, occurs in lymphocytes, and may modulate immune response.
AB - Steroid metabolism was investigated in cultured human B-lymphoblastoid cells (B-LCL), and peripheral blood T and B cells. Gene expression was examined by reverse-transcription polymerase chain reaction amplification (RT-PCR). Appropriate sized transcripts were detected in both cultured and fresh peripheral lymphocytes for CYP11A, CYP17, HSD11L (11β-hydroxysteroid dehydrogenase I), HSD17B1 (17β-hydroxysteroid dehydrogenase type I) and SRD5A1 (5α-reductase I). B-LCL, but not T and B cells, expressed CYP11B. There was minimal expression of HSD3B1 and HSD3B2 (3β-hydroxysteroid dehydrogenase I and II) in B-LCL and T cells. Transcripts for CYP19 and HSD11K were not detected. Corresponding enzymatic activity was detectable only for 17-hydroxysteroid dehydrogenase and 5α-reductase, respectively producing testosterone and 5α-dihydrotestosterone. Steroid identities were confirmed by gas chromatography/mass spectrometry (GC/MS). One metabolite thought to be deoxycorticosterone was identified by GC/MS as 6α-hydroxypregnanolone. It was concluded that sex hormone metabolism, including androgen synthesis, occurs in lymphocytes, and may modulate immune response.
KW - Androgens
KW - Lymphocytes
KW - Steroid 17 hydroxysteroid dehydrogenase
KW - Steroid 5 alpha reductase
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U2 - 10.1016/S0303-7207(98)00052-5
DO - 10.1016/S0303-7207(98)00052-5
M3 - Article
C2 - 9685215
AN - SCOPUS:0032536722
SN - 0303-7207
VL - 138
SP - 61
EP - 69
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -