Promoter and enhancer elements from the rat elastase I gene function independently of each other and of heterologous enhancers.

D. M. Ornitz, Robert E Hammer, B. L. Davison, R. L. Brinster, R. D. Palmiter

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An elastase-human growth hormone (hGH) fusion gene containing 205 base pairs of elastase 5' flanking region is expressed exclusively in pancreatic acinar cells of transgenic mice. This paper shows that the promoter region (-72 to +8) and the enhancer (-205 to -73) function independently of each other. The elastase enhancer can activate the heterologous mouse metallothionein gene and the hGH gene promoters; conversely, enhancers from the thymocyte-specific murine leukemia virus MCF13 and the metal regulatory elements from the metallothionein gene can activate the elastase promoter in a variety of cell types. Combinations of immunoglobulin and elastase enhancers with a heterologous promoter and the hGH gene result in expression in all of the tissues predicted by the sum of each enhancer acting alone. Thus these enhancer elements act independently of each other, suggesting that they do not have silencing activity in cells in which they are normally inactive.

Original languageEnglish (US)
Pages (from-to)3466-3472
Number of pages7
JournalMolecular and Cellular Biology
Volume7
Issue number10
StatePublished - Oct 1987

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Pancreatic Elastase
Human Growth Hormone
Metallothionein
Genes
Murine Leukemia Viruses
Acinar Cells
5' Flanking Region
Gene Fusion
Thymocytes
Genetic Promoter Regions
Base Pairing
Transgenic Mice
Immunoglobulins
Metals

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Promoter and enhancer elements from the rat elastase I gene function independently of each other and of heterologous enhancers. / Ornitz, D. M.; Hammer, Robert E; Davison, B. L.; Brinster, R. L.; Palmiter, R. D.

In: Molecular and Cellular Biology, Vol. 7, No. 10, 10.1987, p. 3466-3472.

Research output: Contribution to journalArticle

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