Pronounced cohabitation of active immunoglobulin genes from three different chromosomes in transcription factories during maximal antibody synthesis

Sung Kyun Park, Yougui Xiang, Xin Feng, William T. Garrard

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

To understand the relationships between nuclear organization and gene expression in a model system, we employed three-dimensional imaging and chromatin immunoprecipitation (ChIP)-chromosome conformation capture (3C) techniques to investigate the topographies of the immunoglobulin (Ig) genes and transcripts during B-cell development. Remarkably, in plasma cells, when antibody synthesis peaks, active Ig genes residing on three different chromosomes exhibit pronounced colocalizations in transcription factories, often near the nuclear periphery, and display trans-chromosomal enhancer interactions, and their transcripts frequently share interchromatin trafficking channels. Conceptually, these features of nuclear organization maximize coordinated transcriptional and transcript trafficking control for potentiating the optimal cytoplasmic assembly of the resulting translation products into protein multimers.

Original languageEnglish (US)
Pages (from-to)1159-1164
Number of pages6
JournalGenes and Development
Volume28
Issue number11
DOIs
StatePublished - Jun 1 2014

Keywords

  • Enhancers
  • Immunoglobulin genes
  • Long-range interactions
  • Nuclear organization
  • RNA trafficking
  • Transcription factories

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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