TY - JOUR
T1 - Pronounced cohabitation of active immunoglobulin genes from three different chromosomes in transcription factories during maximal antibody synthesis
AU - Park, Sung Kyun
AU - Xiang, Yougui
AU - Feng, Xin
AU - Garrard, William T.
PY - 2014/6/1
Y1 - 2014/6/1
N2 - To understand the relationships between nuclear organization and gene expression in a model system, we employed three-dimensional imaging and chromatin immunoprecipitation (ChIP)-chromosome conformation capture (3C) techniques to investigate the topographies of the immunoglobulin (Ig) genes and transcripts during B-cell development. Remarkably, in plasma cells, when antibody synthesis peaks, active Ig genes residing on three different chromosomes exhibit pronounced colocalizations in transcription factories, often near the nuclear periphery, and display trans-chromosomal enhancer interactions, and their transcripts frequently share interchromatin trafficking channels. Conceptually, these features of nuclear organization maximize coordinated transcriptional and transcript trafficking control for potentiating the optimal cytoplasmic assembly of the resulting translation products into protein multimers.
AB - To understand the relationships between nuclear organization and gene expression in a model system, we employed three-dimensional imaging and chromatin immunoprecipitation (ChIP)-chromosome conformation capture (3C) techniques to investigate the topographies of the immunoglobulin (Ig) genes and transcripts during B-cell development. Remarkably, in plasma cells, when antibody synthesis peaks, active Ig genes residing on three different chromosomes exhibit pronounced colocalizations in transcription factories, often near the nuclear periphery, and display trans-chromosomal enhancer interactions, and their transcripts frequently share interchromatin trafficking channels. Conceptually, these features of nuclear organization maximize coordinated transcriptional and transcript trafficking control for potentiating the optimal cytoplasmic assembly of the resulting translation products into protein multimers.
KW - Enhancers
KW - Immunoglobulin genes
KW - Long-range interactions
KW - Nuclear organization
KW - RNA trafficking
KW - Transcription factories
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U2 - 10.1101/gad.237479.114
DO - 10.1101/gad.237479.114
M3 - Article
C2 - 24888587
AN - SCOPUS:84901779165
SN - 0890-9369
VL - 28
SP - 1159
EP - 1164
JO - Genes and Development
JF - Genes and Development
IS - 11
ER -