Properties of noninfectious and transforming viruses released by murine sarcoma virus induced hamster tumor cells

A. F. Gazdar, E. Russell, P. S. Sarma, P. S. Sarin, W. Hall, H. C. Chopra

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The cell culture lines HTG2 and HTG3 were established from a transplantable hamster tumor induced by a murine sarcoma virus (strain Gz MSV) after 17 and 60 in vivo passages, respectively. The viruses released by these two cell lines markedly differ in morphology, antigenic composition, infectivity, transforming ability, and enzymatic activity. HTG2 virions contained the sarcoma genome but were noninfectious for mouse and hamster cells (S + H virus). HTG3 virions transformed hamster but not mouse cells. Whereas HTG2 cells and its virus contained murine type C virus gs 1 antigen, all HTG3 clonal lines expressed both murine and hamster type C virus gs 1 antigens. The RNA dependent DNA polymerase activity of HTG2 virus was very low, whereas that of HTG3 virus was relatively high. HTG2 virions contained electron lucent centers only. HTG3 virus consisted of the expected mixture of virions with electron dense and electron lucent centers. Many broken or incomplete virions were present in both viruses. HTG2 virus is a noninfectious 'defective' sarcoma virus without detectable helper virus. Data obtained in these experiments suggest that HTG3 virus is a hamster type C virus pseudotype of Gz MSV (Gz MSV [HaLV]). The genome of Gz MSV is capable of antigenic expression in heterologous cells and in the presence of heterologous viruses. Attempts to chemically activate hamster type C virus (HaLV) from HTG2 cells were unsuccessful. The HTG1 cell culture line, established from another Gz MSV induced hamster tumor, initially released a virus indistinguishable from the HTG2 virus. After in vitro passage, spontaneous activation of HaLV occurred in HTG1 cells, and the resultant Gz MSV (HaLV) had properties similar to those of the HTG3 virus.

Original languageEnglish (US)
Pages (from-to)931-936
Number of pages6
JournalJournal of Virology
Volume12
Issue number4
StatePublished - 1973

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Murine Sarcoma Viruses
sarcoma
hamsters
Cricetinae
Viruses
viruses
mice
Neoplasms
Virion
virion
neoplasm cells
Electrons
Cell Line
Sarcoma
electrons
cells
Cell Culture Techniques
Genome
Helper Viruses
cell culture

ASJC Scopus subject areas

  • Immunology

Cite this

Gazdar, A. F., Russell, E., Sarma, P. S., Sarin, P. S., Hall, W., & Chopra, H. C. (1973). Properties of noninfectious and transforming viruses released by murine sarcoma virus induced hamster tumor cells. Journal of Virology, 12(4), 931-936.

Properties of noninfectious and transforming viruses released by murine sarcoma virus induced hamster tumor cells. / Gazdar, A. F.; Russell, E.; Sarma, P. S.; Sarin, P. S.; Hall, W.; Chopra, H. C.

In: Journal of Virology, Vol. 12, No. 4, 1973, p. 931-936.

Research output: Contribution to journalArticle

Gazdar, AF, Russell, E, Sarma, PS, Sarin, PS, Hall, W & Chopra, HC 1973, 'Properties of noninfectious and transforming viruses released by murine sarcoma virus induced hamster tumor cells', Journal of Virology, vol. 12, no. 4, pp. 931-936.
Gazdar, A. F. ; Russell, E. ; Sarma, P. S. ; Sarin, P. S. ; Hall, W. ; Chopra, H. C. / Properties of noninfectious and transforming viruses released by murine sarcoma virus induced hamster tumor cells. In: Journal of Virology. 1973 ; Vol. 12, No. 4. pp. 931-936.
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