ProSight Annotator: Complete control and customization of protein entries in UniProt XML files

Joseph B. Greer, Bryan P. Early, Kenneth R. Durbin, Steven M. Patrie, Paul M. Thomas, Neil L. Kelleher, Richard D. LeDuc, Ryan T. Fellers

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The effectiveness of any proteomics database search depends on the theoretical candidate information contained in the protein database. Unfortunately, candidate entries from protein databases such as UniProt rarely contain all the post-translational modifications (PTMs), disulfide bonds, or endogenous cleavages of interest to researchers. These omissions can limit discovery of novel and biologically important proteoforms. Conversely, searching for a specific proteoform becomes a computationally difficult task for heavily modified proteins. Both situations require updates to the database through user-annotated entries. Unfortunately, manually creating properly formatted UniProt Extensible Markup Language (XML) files is tedious and prone to errors. ProSight Annotator solves these issues by providing a graphical interface for adding user-defined features to UniProt-formatted XML files for better informed proteoform searches. It can be downloaded from http://prosightannotator.northwestern.edu.

Original languageEnglish (US)
JournalProteomics
DOIs
StateAccepted/In press - 2022
Externally publishedYes

Keywords

  • bottom-up proteomics
  • post-translational modification
  • proteoforms
  • proteomics software
  • top-down proteomics
  • UniProt

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'ProSight Annotator: Complete control and customization of protein entries in UniProt XML files'. Together they form a unique fingerprint.

Cite this