Protease-activated receptor 1 deletion causes enhanced osteoclastogenesis in response to inflammatory signals through a Notch2-dependent mechanism

Sandra Jastrzebski, Judith Kalinowski, Sehwan Mun, Bongjin Shin, Naga Suresh Adapala, Christian E. Jacome-Galarza, Faryal Mirza, H. Leonardo Aguila, Hicham Drissi, Archana Sanjay, Ernesto Canalis, Sun Kyeong Lee, Joseph A. Lorenzo

Research output: Contribution to journalArticle

Abstract

We found that protease-activated receptor 1 (PAR1) was transiently induced in cultured osteoclast precursor cells. Therefore, we examined the bone phenotype and response to resorptive stimuli of PAR1-deficient (knockout [KO]) mice. Bones and bone marrow-derived cells from PAR1 KO and wild-type (WT) mice were assessed using microcomputed tomography, histomorphometry, in vitro cultures, and RT-PCR. Osteoclastic responses to TNF-a (TNF) challenge in calvaria were analyzed with and without a specific neutralizing Ab to the Notch2-negative regulatory region (N2-NRR Ab). In vivo under homeostatic conditions, there were minimal differences in bone mass or bone cells between PAR1 KO and WT mice. However, PAR1 KO myeloid cells demonstrated enhanced osteoclastogenesis in response to receptor activator of NF-kB ligand (RANKL) or the combination of RANKL and TNF. Strikingly, in vivo osteoclastogenic responses of PAR1 KO mice to TNF were markedly enhanced. We found that N2-NRR Ab reduced TNF-induced osteoclastogenesis in PAR1 KO mice to WT levels without affecting WT responses. Similarly, in vitro N2-NRR Ab reduced RANKL-induced osteoclastogenesis in PAR1 KO cells to WT levels without altering WT responses. We conclude that PAR1 functions to limit Notch2 signaling in responses to RANKL and TNF and moderates osteoclastogenic response to these cytokines. This effect appears, at least in part, to be cell autonomous because enhanced osteoclastogenesis was seen in highly purified PAR1 KO osteoclast precursor cells. It is likely that this pathway is involved in regulating the response of bone to diseases associated with inflammatory signals.

Original languageEnglish (US)
Pages (from-to)105-116
Number of pages12
JournalJournal of Immunology
Volume203
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Jastrzebski, S., Kalinowski, J., Mun, S., Shin, B., Adapala, N. S., Jacome-Galarza, C. E., Mirza, F., Leonardo Aguila, H., Drissi, H., Sanjay, A., Canalis, E., Lee, S. K., & Lorenzo, J. A. (2019). Protease-activated receptor 1 deletion causes enhanced osteoclastogenesis in response to inflammatory signals through a Notch2-dependent mechanism. Journal of Immunology, 203(1), 105-116. https://doi.org/10.4049/jimmunol.1801032