Protease activity of PprI facilitates DNA damage response: Mn(2+)-dependence and substrate sequence-specificity of the proteolytic reaction

Yunguang Wang, Qiang Xu, Huiming Lu, Lin Lin, Liangyan Wang, Hong Xu, Xianyan Cui, Hui Zhang, Tingting Li, Yuejin Hua

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The extremophilic bacterium Deinococcus radiodurans exhibits an extraordinary resistance to ionizing radiation. Previous studies established that a protein named PprI, which exists only in the Deinococcus-Thermus family, acts as a general switch to orchestrate the expression of a number of DNA damage response (DDR) proteins involved in cellular radio-resistance. Here we show that the regulatory mechanism of PprI depends on its Mn(2+)-dependent protease activity toward DdrO, a transcription factor that suppresses DDR genes' expression. Recognition sequence-specificity around the PprI cleavage site is essential for DNA damage repair in vivo. PprI and DdrO mediate a novel DNA damage response pathway differing from the classic LexA-mediated SOS response system found in radiation-sensitive bacterium Escherichia coli. This PprI-mediated pathway in D. radiodurans is indispensable for its extreme radio-resistance and therefore its elucidation significantly advances our understanding of the DNA damage repair mechanism in this amazing organism.

Original languageEnglish (US)
Article numbere0122071
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 26 2015

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Substrate Specificity
DNA damage
DNA Damage
Deinococcus
Peptide Hydrolases
proteinases
Deinococcus radiodurans
DNA
Substrates
radiation resistance
Radio
Deinococcus-Thermus
DNA Repair
Bacteria
Repair
SOS response (genetics)
Thermus
bacteria
Ionizing radiation
Ionizing Radiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Protease activity of PprI facilitates DNA damage response : Mn(2+)-dependence and substrate sequence-specificity of the proteolytic reaction. / Wang, Yunguang; Xu, Qiang; Lu, Huiming; Lin, Lin; Wang, Liangyan; Xu, Hong; Cui, Xianyan; Zhang, Hui; Li, Tingting; Hua, Yuejin.

In: PLoS One, Vol. 10, No. 3, e0122071, 26.03.2015.

Research output: Contribution to journalArticle

Wang, Yunguang ; Xu, Qiang ; Lu, Huiming ; Lin, Lin ; Wang, Liangyan ; Xu, Hong ; Cui, Xianyan ; Zhang, Hui ; Li, Tingting ; Hua, Yuejin. / Protease activity of PprI facilitates DNA damage response : Mn(2+)-dependence and substrate sequence-specificity of the proteolytic reaction. In: PLoS One. 2015 ; Vol. 10, No. 3.
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