Protease inhibitor therapy improves protein catabolism in prepubertal children with HIV infection

Dana S. Hardin, Kenneth J. Ellis, Julie Rice, Marilyn E. Doyle

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Past studies in adults have reported that loss of lean tissue mass (LTM) is associated with accelerated rates of protein catabolism. To date, studies of protein kinetics from pediatric patients infected by HIV have not been published; however, poor linear growth and weight loss are well-documented. The first aim of this study was to test the hypothesis that protein catabolism is high in pediatric patients with HIV. Protease inhibitors (PI) have proven to be effective therapy for pediatric HIV patients. One action of these drugs is that of lowering the viral burden, and several studies suggest that these drugs result in increased growth and weight velocity. Our second aim was to determine whether PI therapy improves protein catabolism. Methods: We studied eight children infected with HIV (ages 2.9-6.2 years, Tanner stage I, CD4 counts 100,000-300,000, 5 F/3 M) and eight healthy age- and gender-matched controls. Measures of protein turnover were conducted using the stable isotope [1-13C]leucine. Body composition was measured by dual X-ray absorptiometry (DXA) scan for determination of LTM, and indirect calorimetry for measurement of resting energy expenditure. Children with HIV infection were studied at baseline and after 6 weeks of PI therapy; control children were studied only once. Results: Protein catabolism, represented as leucine rate of appearance (Ra) in the fasted state, was higher in the HIV-infected children at baseline compared to control children. After 6 weeks of PI therapy, leucine Ra decreased, but not to the range found in control children. Leucine Ra correlated with viral burden. LTM significantly improved in all patients. Conclusion: These results suggest that similar to HIV-infected adults, HIV-infected children have higher than normal protein catabolism. Furthermore, our measures suggest that short-term PI therapy results in improved protein catabolism and LTM.

Original languageEnglish (US)
Pages (from-to)321-325
Number of pages5
JournalJournal of Pediatric Endocrinology and Metabolism
Volume17
Issue number3
StatePublished - 2004

Fingerprint

Protease Inhibitors
HIV Infections
HIV
Leucine
Proteins
Therapeutics
Pediatrics
Viral Load
Indirect Calorimetry
Photon Absorptiometry
CD4 Lymphocyte Count
Growth
Body Composition
Isotopes
Pharmaceutical Preparations
Energy Metabolism
Weight Loss
Weights and Measures

Keywords

  • Children
  • Growth
  • HIV
  • Nutrition
  • Protein turnover
  • Weight

ASJC Scopus subject areas

  • Endocrinology
  • Pediatrics, Perinatology, and Child Health

Cite this

Protease inhibitor therapy improves protein catabolism in prepubertal children with HIV infection. / Hardin, Dana S.; Ellis, Kenneth J.; Rice, Julie; Doyle, Marilyn E.

In: Journal of Pediatric Endocrinology and Metabolism, Vol. 17, No. 3, 2004, p. 321-325.

Research output: Contribution to journalArticle

Hardin, Dana S. ; Ellis, Kenneth J. ; Rice, Julie ; Doyle, Marilyn E. / Protease inhibitor therapy improves protein catabolism in prepubertal children with HIV infection. In: Journal of Pediatric Endocrinology and Metabolism. 2004 ; Vol. 17, No. 3. pp. 321-325.
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abstract = "Past studies in adults have reported that loss of lean tissue mass (LTM) is associated with accelerated rates of protein catabolism. To date, studies of protein kinetics from pediatric patients infected by HIV have not been published; however, poor linear growth and weight loss are well-documented. The first aim of this study was to test the hypothesis that protein catabolism is high in pediatric patients with HIV. Protease inhibitors (PI) have proven to be effective therapy for pediatric HIV patients. One action of these drugs is that of lowering the viral burden, and several studies suggest that these drugs result in increased growth and weight velocity. Our second aim was to determine whether PI therapy improves protein catabolism. Methods: We studied eight children infected with HIV (ages 2.9-6.2 years, Tanner stage I, CD4 counts 100,000-300,000, 5 F/3 M) and eight healthy age- and gender-matched controls. Measures of protein turnover were conducted using the stable isotope [1-13C]leucine. Body composition was measured by dual X-ray absorptiometry (DXA) scan for determination of LTM, and indirect calorimetry for measurement of resting energy expenditure. Children with HIV infection were studied at baseline and after 6 weeks of PI therapy; control children were studied only once. Results: Protein catabolism, represented as leucine rate of appearance (Ra) in the fasted state, was higher in the HIV-infected children at baseline compared to control children. After 6 weeks of PI therapy, leucine Ra decreased, but not to the range found in control children. Leucine Ra correlated with viral burden. LTM significantly improved in all patients. Conclusion: These results suggest that similar to HIV-infected adults, HIV-infected children have higher than normal protein catabolism. Furthermore, our measures suggest that short-term PI therapy results in improved protein catabolism and LTM.",
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