Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli

Wayne C. Lai, Michael Bennett, Brian E. Gordon, Steven P. Pakes

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalVaccine
Volume12
Issue number4
DOIs
StatePublished - 1994

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Keywords

  • Escherichia coli
  • Mycoplasma pulmonis
  • fusion protein
  • lysogen

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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