Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli

Wayne C. Lai; Michael Bennett; Brian E. Gordon; Steven P. Pakes

doi:10.1016/0264-410X(94)90091-4

Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli

Wayne C. Lai, Michael Bennett, Brian E. Gordon, Steven P. Pakes

Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.

Original language	English (US)
Pages (from-to)	291-298
Number of pages	8
Journal	Vaccine
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/0264-410X(94)90091-4
State	Published - 1994

Keywords

Escherichia coli
Mycoplasma pulmonis
fusion protein
lysogen

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0264-410X(94)90091-4

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title = "Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli",

abstract = "A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.",

keywords = "Escherichia coli, Mycoplasma pulmonis, fusion protein, lysogen",

author = "Lai, {Wayne C.} and Michael Bennett and Gordon, {Brian E.} and Pakes, {Steven P.}",

note = "Funding Information: This work wass upporteidn partb yP ublicH ealthS ervice grants RR00890 and RR08552 from the National Institutes of Health. The authors thank David SteutermanGn,e offreyT addaa ndSu-In Ho for excellent technicaal ssistancaen d BonnieM endroand J. Harvey Harris for outstandinsge cretariahle lp.T heyalso thank Drs RaymondJ. MacDonalda nd Galvin Swift for the suggestiotno shearM P DNA into small piecest o deal with TGA stop codons,a nd Dr Victor K. Lin for his technicaal dvicec oncerningth e cloningp rocedureW. e also thank Dr Ping Chuan Hu (Universityo f North CarolinaC, hapeHl ill, NC) for sequencintgh ei nsertD NA.",

year = "1994",

doi = "10.1016/0264-410X(94)90091-4",

language = "English (US)",

volume = "12",

pages = "291--298",

journal = "Vaccine",

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AU - Lai, Wayne C.

AU - Bennett, Michael

AU - Gordon, Brian E.

AU - Pakes, Steven P.

N1 - Funding Information: This work wass upporteidn partb yP ublicH ealthS ervice grants RR00890 and RR08552 from the National Institutes of Health. The authors thank David SteutermanGn,e offreyT addaa ndSu-In Ho for excellent technicaal ssistancaen d BonnieM endroand J. Harvey Harris for outstandinsge cretariahle lp.T heyalso thank Drs RaymondJ. MacDonalda nd Galvin Swift for the suggestiotno shearM P DNA into small piecest o deal with TGA stop codons,a nd Dr Victor K. Lin for his technicaal dvicec oncerningth e cloningp rocedureW. e also thank Dr Ping Chuan Hu (Universityo f North CarolinaC, hapeHl ill, NC) for sequencintgh ei nsertD NA.

PY - 1994

Y1 - 1994

N2 - A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.

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KW - Escherichia coli

KW - Mycoplasma pulmonis

KW - fusion protein

KW - lysogen

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Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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