Abstract
A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 291-298 |
| Number of pages | 8 |
| Journal | Vaccine |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1994 |
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Keywords
- Escherichia coli
- fusion protein
- lysogen
- Mycoplasma pulmonis
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli. / Lai, Wayne C.; Bennett, Michael; Gordon, Brian E.; Pakes, Steven P.
In: Vaccine, Vol. 12, No. 4, 1994, p. 291-298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli
AU - Lai, Wayne C.
AU - Bennett, Michael
AU - Gordon, Brian E.
AU - Pakes, Steven P.
PY - 1994
Y1 - 1994
N2 - A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.
AB - A construct of the Mycoplasma pulmonis (MP) genomic library, using randomly sheared DNA, was cloned in λgt11 and transfected into C600 Escherichia coli organisms. Clones of E. coli expressing a fusion protein reactive with anti-MP and monospecific serum were transferred orally or intravenously into Balb/c mice. Expression of the fusion protein was induced by adding isopropyl-β-D-thiogalactopyranoside to the drinking water. This vaccination protocol led to local and systemic antibody formation, to generation of immune lymphocytes and to protection against large numbers of virulent MP organisms. This approach might be generally successful in preventing infectious disease.
KW - Escherichia coli
KW - fusion protein
KW - lysogen
KW - Mycoplasma pulmonis
UR - http://www.scopus.com/inward/record.url?scp=0028212271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028212271&partnerID=8YFLogxK
U2 - 10.1016/0264-410X(94)90091-4
DO - 10.1016/0264-410X(94)90091-4
M3 - Article
C2 - 8178549
AN - SCOPUS:0028212271
VL - 12
SP - 291
EP - 298
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 4
ER -