TY - JOUR
T1 - Protection of rabbits against experimental pasteurellosis by a streptomycin-dependent Pasteurella multocida serotype 3:A live mutant vaccine
AU - Lu, Y. S.
AU - Pakes, S. P.
PY - 1981
Y1 - 1981
N2 - Pasteurella multocida (serotype 3:A) was isolated from rabbit with clinical signs of suppurative rhinitis. This P. multocida strain was mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine to obtain a genetically stable streptomycin-dependent mutant, from which a live vaccine was prepared. Pasteurella-free rabbits were inoculated intranasally three times at weekly intervals and challenged intranasally with a virulent serotype 3:A rabbit P. multocida isolate 2 weeks after the third vaccination. The rabbits were killed 2 to 3 weeks later. The vaccine did not cause clinical disease, death, or gross or microscopic lesions. Furthermore, the vaccine protected the challenge rabbits from developing clinical disease, death, and gross lesions. However, mild focal lung lesions were noted in several of the vaccinated-challenged animals. In contrast, nonvaccinated-challenged rabbits developed pyrexia and anorexia. Furthermore, three of four of these rabbits died with severe gross lesions including pyothorax, suppurative pericarditis, and fibrinopurulent pneumonia. Microscopically, the four nonvaccinated rabbits had moderate to severe suppurative pneumonia and mild to moderate suppurative rhinitis, and two had mild tympanitis. The mutant vaccine did not appear to colonize the nasal cavities. The vaccine prevented the colonization of the virulent challenge organism in lungs, liver, spleen, genital tracts, and blood, but not the nasal cavities.
AB - Pasteurella multocida (serotype 3:A) was isolated from rabbit with clinical signs of suppurative rhinitis. This P. multocida strain was mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine to obtain a genetically stable streptomycin-dependent mutant, from which a live vaccine was prepared. Pasteurella-free rabbits were inoculated intranasally three times at weekly intervals and challenged intranasally with a virulent serotype 3:A rabbit P. multocida isolate 2 weeks after the third vaccination. The rabbits were killed 2 to 3 weeks later. The vaccine did not cause clinical disease, death, or gross or microscopic lesions. Furthermore, the vaccine protected the challenge rabbits from developing clinical disease, death, and gross lesions. However, mild focal lung lesions were noted in several of the vaccinated-challenged animals. In contrast, nonvaccinated-challenged rabbits developed pyrexia and anorexia. Furthermore, three of four of these rabbits died with severe gross lesions including pyothorax, suppurative pericarditis, and fibrinopurulent pneumonia. Microscopically, the four nonvaccinated rabbits had moderate to severe suppurative pneumonia and mild to moderate suppurative rhinitis, and two had mild tympanitis. The mutant vaccine did not appear to colonize the nasal cavities. The vaccine prevented the colonization of the virulent challenge organism in lungs, liver, spleen, genital tracts, and blood, but not the nasal cavities.
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U2 - 10.1128/iai.34.3.1018-1024.1981
DO - 10.1128/iai.34.3.1018-1024.1981
M3 - Article
C2 - 7333661
AN - SCOPUS:0019790613
SN - 0019-9567
VL - 34
SP - 1018
EP - 1024
JO - Infection and immunity
JF - Infection and immunity
IS - 3
ER -