Protein arrays I: Antibody arrays

Yulin Yuan, Zuan Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, Tianfu Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Antibody arrays represent one of the very early protein array systems where antibodies are used to capture and detect target proteins in a high-throughput platform. The development of high-quality antibodies, nanomaterial-based novel detection probes, as well as innovative imaging technologies and computational tools has tremendously improved the sensitivity, specificity, and robustness of antibody arrays during the past decade. In this protocol we will incorporate the most updated innovations and developments of antibody arrays into the step-by-step experimental procedures. This includes antibody printing, sample preparation, array detection, as well as imaging and data analysis. Antibody array could be used for cytokine profiling or mapping of phosphorylation, glycosylation, or other post-translational modifications of target proteins.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages261-269
Number of pages9
Volume1654
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1654
ISSN (Print)1064-3745

Keywords

  • Antibody array
  • Biomarkers
  • Cytokine profiling
  • Glycome mapping
  • Kinome mapping

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Yuan, Y., Lin, Z. T., Wang, H., Hong, X., Heon, M., & Wu, T. (2017). Protein arrays I: Antibody arrays. In Methods in Molecular Biology (Vol. 1654, pp. 261-269). (Methods in Molecular Biology; Vol. 1654). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7231-9_19