Protein arrays II: Antigen arrays

Yulin Yuan, Hongting Wang, Zuan Tao Lin, Xia Hong, Mikala Heon, Tianfu Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Antigen arrays are fabricated using various antigens such as DNA, histones, synthetic peptides, recombinant proteins, or cell extracts to detect autoantibodies in autoimmune diseases, alloantibodies in transplantation, drug-induced antibodies or cancer-induced antibodies in blood or cell culture supernatant. In this protocol, we will provide a step-by-step executable procedure to perform antigen arrays, including antigen preparation and printing, blocking, sample loading, array detection, imaging, and data analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages271-277
Number of pages7
Volume1654
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1654
ISSN (Print)1064-3745

Fingerprint

Protein Array Analysis
Antigens
Isoantibodies
Printing
Antibodies
Cell Extracts
Recombinant Proteins
Autoantibodies
Histones
Autoimmune Diseases
Blood Cells
Cell Culture Techniques
Transplantation
Peptides
DNA
Pharmaceutical Preparations
Neoplasms

Keywords

  • Antigen arrays
  • Autoantibody profiling
  • Biomarker

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yuan, Y., Wang, H., Lin, Z. T., Hong, X., Heon, M., & Wu, T. (2017). Protein arrays II: Antigen arrays. In Methods in Molecular Biology (Vol. 1654, pp. 271-277). (Methods in Molecular Biology; Vol. 1654). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7231-9_20

Protein arrays II : Antigen arrays. / Yuan, Yulin; Wang, Hongting; Lin, Zuan Tao; Hong, Xia; Heon, Mikala; Wu, Tianfu.

Methods in Molecular Biology. Vol. 1654 Humana Press Inc., 2017. p. 271-277 (Methods in Molecular Biology; Vol. 1654).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yuan, Y, Wang, H, Lin, ZT, Hong, X, Heon, M & Wu, T 2017, Protein arrays II: Antigen arrays. in Methods in Molecular Biology. vol. 1654, Methods in Molecular Biology, vol. 1654, Humana Press Inc., pp. 271-277. https://doi.org/10.1007/978-1-4939-7231-9_20
Yuan Y, Wang H, Lin ZT, Hong X, Heon M, Wu T. Protein arrays II: Antigen arrays. In Methods in Molecular Biology. Vol. 1654. Humana Press Inc. 2017. p. 271-277. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7231-9_20
Yuan, Yulin ; Wang, Hongting ; Lin, Zuan Tao ; Hong, Xia ; Heon, Mikala ; Wu, Tianfu. / Protein arrays II : Antigen arrays. Methods in Molecular Biology. Vol. 1654 Humana Press Inc., 2017. pp. 271-277 (Methods in Molecular Biology).
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