Protein-coated poly(L-lactic acid) fibers provide a substrate for differentiation of human skeletal muscle cells

Elizabeth M. Cronin, Frederick A. Thurmond, Rhonda Bassel-Duby, R. Sanders Williams, Woodring E. Wright, Kevin D. Nelson, Harold R. Garner

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Tissue engineering represents a potential method for repairing damaged skeletal muscle tissue. Extracellular matrix (ECM) proteins were evaluated for their ability to aid in cell attachment, whereas a poly(L-lactic acid) (PLLA) fiber scaffold was tested as a substrate for the differentiation of human skeletal muscle cells. In comparison to uncoated or gelatin-coated PLLA films, cell attachment increased significantly (p < 0.001) on PLLA films coated with ECM gel, fibronectin, or laminin. Myoblasts differentiated into multinucleated myofibers on ECM gel-coated PLLA fibers, and expressed muscle markers such as myosin and α-actinin. Oligonucleotide microarray analysis showed similar gene expression profiles for human skeletal muscle cells on ECM gel-coated PLLA fibers as to that observed for myofibers on tissue culture plates. Therefore, PLLA fibers coated with ECM proteins provide a scaffold for the development of skeletal muscle tissue for tissue engineering and cell transplantation applications.

Original languageEnglish (US)
Pages (from-to)373-381
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume69
Issue number3
DOIs
StatePublished - Jun 1 2004

Keywords

  • Differentiation
  • Extracellular matrix proteins
  • Poly L-lactic acid fiber scaffold
  • Skeletal muscle
  • Tissue engineering

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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