Abstract
Tissue engineering represents a potential method for repairing damaged skeletal muscle tissue. Extracellular matrix (ECM) proteins were evaluated for their ability to aid in cell attachment, whereas a poly(L-lactic acid) (PLLA) fiber scaffold was tested as a substrate for the differentiation of human skeletal muscle cells. In comparison to uncoated or gelatin-coated PLLA films, cell attachment increased significantly (p < 0.001) on PLLA films coated with ECM gel, fibronectin, or laminin. Myoblasts differentiated into multinucleated myofibers on ECM gel-coated PLLA fibers, and expressed muscle markers such as myosin and α-actinin. Oligonucleotide microarray analysis showed similar gene expression profiles for human skeletal muscle cells on ECM gel-coated PLLA fibers as to that observed for myofibers on tissue culture plates. Therefore, PLLA fibers coated with ECM proteins provide a scaffold for the development of skeletal muscle tissue for tissue engineering and cell transplantation applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 373-381 |
| Number of pages | 9 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 69 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 2004 |
Keywords
- Differentiation
- Extracellular matrix proteins
- Poly L-lactic acid fiber scaffold
- Skeletal muscle
- Tissue engineering
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys