Protein Domain of Unknown Function DUF1023 is an α/β hydrolase

Mingzhu Zheng; Krzysztof Ginalski; Leszek Rychlewski; Nick V. Grishin

doi:10.1002/prot.20388

Protein Domain of Unknown Function DUF1023 is an α/β hydrolase

Mingzhu Zheng, Krzysztof Ginalski, Leszek Rychlewski, Nick V. Grishin

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue: D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and α/β hydrolases. DUF1023 proteins conserve the core secondary structures in α/β hydrolase fold, and share similar catalytic machinery as that of α/β hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Proteins: Structure, Function and Genetics
Volume	59
Issue number	1
DOIs	https://doi.org/10.1002/prot.20388
State	Published - Apr 1 2005

Keywords

Actinobacteria
Carboxylesterase
Fungal lipase
Genomic context
Structure prediction
Triad

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

Access to Document

10.1002/prot.20388

Cite this

@article{f95757300ad44f4ab8809087c6eb3908,

title = "Protein Domain of Unknown Function DUF1023 is an α/β hydrolase",

abstract = "Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue: D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and α/β hydrolases. DUF1023 proteins conserve the core secondary structures in α/β hydrolase fold, and share similar catalytic machinery as that of α/β hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.",

keywords = "Actinobacteria, Carboxylesterase, Fungal lipase, Genomic context, Structure prediction, Triad",

author = "Mingzhu Zheng and Krzysztof Ginalski and Leszek Rychlewski and Grishin, {Nick V.}",

year = "2005",

month = apr,

day = "1",

doi = "10.1002/prot.20388",

language = "English (US)",

volume = "59",

pages = "1--6",

journal = "Proteins: Structure, Function and Genetics",

issn = "0887-3585",

publisher = "Wiley-Liss Inc.",

number = "1",

}

TY - JOUR

T1 - Protein Domain of Unknown Function DUF1023 is an α/β hydrolase

AU - Zheng, Mingzhu

AU - Ginalski, Krzysztof

AU - Rychlewski, Leszek

AU - Grishin, Nick V.

PY - 2005/4/1

Y1 - 2005/4/1

N2 - Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue: D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and α/β hydrolases. DUF1023 proteins conserve the core secondary structures in α/β hydrolase fold, and share similar catalytic machinery as that of α/β hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.

AB - Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue: D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and α/β hydrolases. DUF1023 proteins conserve the core secondary structures in α/β hydrolase fold, and share similar catalytic machinery as that of α/β hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.

KW - Actinobacteria

KW - Carboxylesterase

KW - Fungal lipase

KW - Genomic context

KW - Structure prediction

KW - Triad

UR - http://www.scopus.com/inward/record.url?scp=14644420835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644420835&partnerID=8YFLogxK

U2 - 10.1002/prot.20388

DO - 10.1002/prot.20388

M3 - Article

C2 - 15688435

AN - SCOPUS:14644420835

SN - 0887-3585

VL - 59

SP - 1

EP - 6

JO - Proteins: Structure, Function and Genetics

JF - Proteins: Structure, Function and Genetics

IS - 1

ER -

Protein Domain of Unknown Function DUF1023 is an α/β hydrolase

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this