Protein Domain of Unknown Function DUF1023 is an α/β hydrolase

Mingzhu Zheng, Krzysztof Ginalski, Leszek Rychlewski, Nick V. Grishin

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue: D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and α/β hydrolases. DUF1023 proteins conserve the core secondary structures in α/β hydrolase fold, and share similar catalytic machinery as that of α/β hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalProteins: Structure, Function and Genetics
Volume59
Issue number1
DOIs
StatePublished - Apr 1 2005

Keywords

  • Actinobacteria
  • Carboxylesterase
  • Fungal lipase
  • Genomic context
  • Structure prediction
  • Triad

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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