Protein import into yeast mitochondria: The inner membrane import site protein ISP45 is the MPI1 gene product

Martin Horst, Paul Jenö, Nafsika G. Kronidou, Luca Bolliger, Wolfgang Oppliger, Philipp Scherer, Ute Manning-Krieg, Thomas Jascur, Gottfried Schatz

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Protein import across both mitochondrial membranes is mediated by the cooperation of two distinct protein transport systems, one in the outer and the other in the inner membrane. Previously we described a 45 kDa yeast mitochondrial inner membrane protein (ISP45) that can be cross-linked to a partially translocated precursor protein. We have now purified ISP45 to homogeneity and identified it as the product of the nuclear MPI1 gene. Identity of ISP45 with the MPI1 gene product was shown by microsequencing of three tryptic ISP45 peptides and by demonstrating that an antibody against an Mpi1p-β-galactosidase fusion protein specifically recognizes ISP45. Antibodies monospecific for ISP45 inhibited protein import into rightside-out mitochondrial inner membrane vesicles, but not into intact mitochondria. On solubilizing mitochondria, ISP45 was rapidly converted to a 40 kDa proteolytic fragment unless mitochondria were first denatured with trichloroacetic acid. The combined genetic and biochemical evidence identifies ISP45/Mpi1p as a component of the protein import system of the yeast mitochondrial inner membrane.

Original languageEnglish (US)
Pages (from-to)3035-3041
Number of pages7
JournalEMBO Journal
Volume12
Issue number8
StatePublished - Jan 1 1993

    Fingerprint

Keywords

  • Mitochondrial inner membrane
  • Protein translocation/submitochondrial
  • Vesicles
  • Yeast

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Horst, M., Jenö, P., Kronidou, N. G., Bolliger, L., Oppliger, W., Scherer, P., Manning-Krieg, U., Jascur, T., & Schatz, G. (1993). Protein import into yeast mitochondria: The inner membrane import site protein ISP45 is the MPI1 gene product. EMBO Journal, 12(8), 3035-3041.