Protein import into yeast mitochondria

The inner membrane import site protein ISP45 is the MPI1 gene product

Martin Horst, Paul Jenö, Nafsika G. Kronidou, Luca Bolliger, Wolfgang Oppliger, Philipp Scherer, Ute Manning-Krieg, Thomas Jascur, Gottfried Schatz

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Protein import across both mitochondrial membranes is mediated by the cooperation of two distinct protein transport systems, one in the outer and the other in the inner membrane. Previously we described a 45 kDa yeast mitochondrial inner membrane protein (ISP45) that can be cross-linked to a partially translocated precursor protein (Scherer et al., 1992). We have now purified ISP45 to homogeneity and identified it as the product of the nuclear MPI1 gene. Identity of ISP45 with the MPI1 gene product was shown by microsequencing of three tryptic ISP45 peptides and by demonstrating that an antibody against an Mpilp-β-galactosidase fusion protein specifically recognizes ISP45. Antibodies monospecific for ISP45 inhibited protein import into right-side-out mitochondrial inner membrane vesicles, but not into intact mitochondria. On solubilizing mitochondria, ISP45 was rapidly converted to a 40 kDa proteolytic fragment unless mitochondria were first denatured with trichloroacetic acid. The combined genetic and biochemical evidence identifies ISP45/Mpilp as a component of the protein import system of the yeast mitochondrial inner membrane.

Original languageEnglish (US)
Pages (from-to)3035-3041
Number of pages7
JournalEMBO Journal
Volume12
Issue number8
StatePublished - 1993

Fingerprint

Mitochondria
Yeast
Mitochondrial Membranes
Genes
Yeasts
Membranes
Proteins
Galactosidases
Trichloroacetic Acid
Protein Precursors
Antibodies
Protein Transport
Molecular Biology
Membrane Proteins
Fusion reactions
Peptides

Keywords

  • Mitochondrial inner membrane
  • Protein translocation
  • Submitochondrial vesicles
  • Yeast

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Horst, M., Jenö, P., Kronidou, N. G., Bolliger, L., Oppliger, W., Scherer, P., ... Schatz, G. (1993). Protein import into yeast mitochondria: The inner membrane import site protein ISP45 is the MPI1 gene product. EMBO Journal, 12(8), 3035-3041.

Protein import into yeast mitochondria : The inner membrane import site protein ISP45 is the MPI1 gene product. / Horst, Martin; Jenö, Paul; Kronidou, Nafsika G.; Bolliger, Luca; Oppliger, Wolfgang; Scherer, Philipp; Manning-Krieg, Ute; Jascur, Thomas; Schatz, Gottfried.

In: EMBO Journal, Vol. 12, No. 8, 1993, p. 3035-3041.

Research output: Contribution to journalArticle

Horst, M, Jenö, P, Kronidou, NG, Bolliger, L, Oppliger, W, Scherer, P, Manning-Krieg, U, Jascur, T & Schatz, G 1993, 'Protein import into yeast mitochondria: The inner membrane import site protein ISP45 is the MPI1 gene product', EMBO Journal, vol. 12, no. 8, pp. 3035-3041.
Horst, Martin ; Jenö, Paul ; Kronidou, Nafsika G. ; Bolliger, Luca ; Oppliger, Wolfgang ; Scherer, Philipp ; Manning-Krieg, Ute ; Jascur, Thomas ; Schatz, Gottfried. / Protein import into yeast mitochondria : The inner membrane import site protein ISP45 is the MPI1 gene product. In: EMBO Journal. 1993 ; Vol. 12, No. 8. pp. 3035-3041.
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