Protein kinase C α is involved in the regulation of AXL receptor tyrosine kinase expression in triple-negative breast cancer cells

Chia Herng Yue, Liang Chih Liu, Erl Shyh Kao, Ho Lin, Li Sung Hsu, Chih Wei Hsu, Yu Yu Lin, Yi Syuan Lin, Jer Yuh Liu, Chia Jen Lee

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

AXL receptor tyrosine kinase is overexpressed in triple-negative breast cancer (TNBC), and has a function in cancer progression and metastases. However, the mechanism underlying AXL gene regulation in TNBC remains unknown. In this study, the involvement of protein kinase C α (PKCα) in the expression of AXL was investigated in human TNBC cells. The microarray data from other studies showed that PKCα is significantly correlated with AXL expression in TNBC cell lines. Tissue array analysis also confirmed their correlation in TNBC. The PKCα inhibitor Go6976 was used to treat MDA-MB-231 and Hs578T TNBC cells, which resulted in decreased expression of AXL and epithelia-mesenchymal transition-related gene vimentin, and decreased cell proliferation. An MZF-1 acidic domain fragment (MZF-1 peptide), which was designed to downregulate PKCα expression, was transfected into the cells and resulted in inhibition of AXL expression. This effect was reversed by co-treatment with the constitutive form of PKCα. Moreover, the downregulation of PKCα was also confirmed by treatment with TAT-fused MZF-1 peptide. Thus, the current study proposes that AXL may be correlated with PKCα-dependent TNBC cells, and could be modulated by MZF-1 peptides.

Original languageEnglish (US)
Pages (from-to)1636-1642
Number of pages7
JournalMolecular Medicine Reports
Volume14
Issue number2
DOIs
StatePublished - Aug 2016

Keywords

  • AXL receptor tyrosine kinase
  • Kinase protein kinase C α
  • Triple-negative breast cancer

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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  • Cite this

    Yue, C. H., Liu, L. C., Kao, E. S., Lin, H., Hsu, L. S., Hsu, C. W., Lin, Y. Y., Lin, Y. S., Liu, J. Y., & Lee, C. J. (2016). Protein kinase C α is involved in the regulation of AXL receptor tyrosine kinase expression in triple-negative breast cancer cells. Molecular Medicine Reports, 14(2), 1636-1642. https://doi.org/10.3892/mmr.2016.5424