Proteomic analysis of mismatch repair-mediated alkylating agent-induced DNA damage response

Xi Chen, Yong Zhao, Guo Min Li, Lin Guo

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Background: Mediating DNA damage-induced apoptosis is an important genome-maintenance function of the mismatch repair (MMR) system. Defects in MMR not only cause carcinogenesis, but also render cancer cells highly resistant to chemotherapeutics, including alkylating agents. To understand the mechanisms of MMR-mediated apoptosis and MMR-deficiency-caused drug resistance, we analyze a model alkylating agent (N-methyl-N'-nitro-N-nitrosoguanidine, MNNG)-induced changes in protein phosphorylation and abundance in two cell lines, the MMR-proficient TK6 and its derivative MMR-deficient MT1.Results: Under an experimental condition that MNNG-induced apoptosis was only observed in MutSα-proficient (TK6), but not in MutSα-deficient (MT1) cells, quantitative analysis of the proteomic data revealed differential expression and phosphorylation of numerous individual proteins and clusters of protein kinase substrates, as well differential activation of response pathways/networks in MNNG-treated TK6 and MT1 cells. Many alterations in TK6 cells are in favor of turning on the apoptotic machinery, while many of those in MT1 cells are to promote cell proliferation and anti-apoptosis.Conclusions: Our work provides novel molecular insights into the mechanism of MMR-mediated DNA damage-induced apoptosis.

Original languageEnglish (US)
Article number37
JournalCell and Bioscience
Volume3
Issue number1
DOIs
StatePublished - Sep 19 2013

Keywords

  • DNA damage response
  • MNNG
  • MSH6
  • SILAC-based quantitative mass spectrometry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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