Proteomic analysis of the anti-cancer effect of 20S-ginsenoside Rg 3 in human colon cancer cell lines

Seo Young Lee, Geun Tae Kim, Si Hun Roh, Jin Su Song, Hie Joon Kim, Soon Sun Hong, Sung Won Kwon, Jeong Hill Park

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

Ginseng is a well known herbal medicine in Asia, and ginsenoside Rg 3 has anti-cancer and various pharmacological effects. In particular, 20S-ginsenoside Rg3 may increase the anti-proliferative effects of chemotherapy. The authors investigated the mechanism of the anti-proliferative effect of 20S-Rg3 at the protein level in HT29 colon cancer cells. MTT, caspase-3 assays, and flow cytometry analysis were performed to determine cytotoxicity and apoptosis, and proteomic analysis was performed by two-dimensional gel electrophoresis and MALDI-TOF/TOF MS, and a database was used to identify protein changes in 20S-Rg3 treated HT29 cells. The proteins identified included down-regulated Rho GDP dissociation inhibitor, up-regulated tropomyosin1, and annexin5 and glutathione s-transferase p1, which are apoptosis associated proteins. The anti-proliferative mechanism of 20S-Rg3 was found to be involved in mitotic inhibition, DNA replication, and repair and growth factor signaling. The findings of this study suggest that the cytotoxicity of 20S-Rg3 in colon cancer is dependent on several mechanisms, including apoptosis.

Original languageEnglish (US)
Pages (from-to)811-816
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number4
DOIs
StatePublished - 2009

Keywords

  • Anti-cancer activity
  • Colon cancer
  • Ginsenoside
  • Proteomics

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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