Proteomic profile of Cryptococcus neoformans biofilm reveals changes in metabolic processes

Lucélia Santi, Walter O. Beys-Da-Silva, Markus Berger, Diego Calzolari, Jorge A. Guimarães, James J. Moresco, John R. Yates

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Cryptococcus neoformans, a pathogenic yeast, causes meningoencephalitis, especially in immunocompromised patients, leading in some cases to death. Microbes in biofilms can cause persistent infections, which are harder to treat. Cryptococcal biofilms are becoming common due to the growing use of brain valves and other medical devices. Using shotgun proteomics we determine the differences in protein abundance between biofilm and planktonic cells. Applying bioinformatic tools, we also evaluated the metabolic pathways involved in biofilm maintenance and protein interactions. Our proteomic data suggest general changes in metabolism, protein turnover, and global stress responses. Biofilm cells show an increase in proteins related to oxidation-reduction, proteolysis, and response to stress and a reduction in proteins related to metabolic process, transport, and translation. An increase in pyruvate-utilizing enzymes was detected, suggesting a shift from the TCA cycle to fermentation-derived energy acquisition. Additionally, we assign putative roles to 33 proteins previously categorized as hypothetical. Many changes in metabolic enzymes were identified in studies of bacterial biofilm, potentially revealing a conserved strategy in biofilm lifestyle.

Original languageEnglish (US)
Pages (from-to)1545-1559
Number of pages15
JournalJournal of Proteome Research
Volume13
Issue number3
DOIs
StatePublished - Mar 7 2014
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Biofilms
Proteomics
Proteins
Proteolysis
Meningoencephalitis
Firearms
Immunocompromised Host
Enzymes
Bioinformatics
Metabolic Networks and Pathways
Computational Biology
Pyruvic Acid
Metabolism
Yeast
Fermentation
Oxidation-Reduction
Life Style
Brain
Yeasts

Keywords

  • biofilm
  • Cryptococcus neoformans
  • metabolic changes
  • resistance
  • shotgun proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Santi, L., Beys-Da-Silva, W. O., Berger, M., Calzolari, D., Guimarães, J. A., Moresco, J. J., & Yates, J. R. (2014). Proteomic profile of Cryptococcus neoformans biofilm reveals changes in metabolic processes. Journal of Proteome Research, 13(3), 1545-1559. https://doi.org/10.1021/pr401075f

Proteomic profile of Cryptococcus neoformans biofilm reveals changes in metabolic processes. / Santi, Lucélia; Beys-Da-Silva, Walter O.; Berger, Markus; Calzolari, Diego; Guimarães, Jorge A.; Moresco, James J.; Yates, John R.

In: Journal of Proteome Research, Vol. 13, No. 3, 07.03.2014, p. 1545-1559.

Research output: Contribution to journalArticle

Santi, L, Beys-Da-Silva, WO, Berger, M, Calzolari, D, Guimarães, JA, Moresco, JJ & Yates, JR 2014, 'Proteomic profile of Cryptococcus neoformans biofilm reveals changes in metabolic processes', Journal of Proteome Research, vol. 13, no. 3, pp. 1545-1559. https://doi.org/10.1021/pr401075f
Santi, Lucélia ; Beys-Da-Silva, Walter O. ; Berger, Markus ; Calzolari, Diego ; Guimarães, Jorge A. ; Moresco, James J. ; Yates, John R. / Proteomic profile of Cryptococcus neoformans biofilm reveals changes in metabolic processes. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 3. pp. 1545-1559.
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