Proteomics profiling of kaimrc1 in comparison to MDA-MB231 and MCF-7

Bandar Alghanem, Rizwan Ali, Atef Nehdi, Hajar Al Zahrani, Abdulelah Altolayyan, Hayat Shaibah, Omar Baz, Alshaimaa Alhallaj, James J. Moresco, Jolene K. Diedrich, John R. Yates, Mohamed Boudjelal

Research output: Contribution to journalReview article

Abstract

Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF-7 and MDA-MB-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT)-labeled technique in conjunction with the phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, splicing machinery, amino acid metabolism, cellular energy, and estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including S-methyl-5-thioadenosine phosphorylase (MTAP), BTB/POZ domain-containing protein (KCTD12), Poly (ADP-ribose) polymerase 1 (PARP 1), and Prelamin-A/C were subjected to western blotting, and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho-and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer.

Original languageEnglish (US)
Article number4328
Pages (from-to)1-15
Number of pages15
JournalInternational journal of molecular sciences
Volume21
Issue number12
DOIs
StatePublished - Jun 2 2020

Keywords

  • Breast cancer
  • Cell lines
  • Mass spectrometry
  • Phosphoproteomic
  • Proteomics
  • TMT

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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  • Cite this

    Alghanem, B., Ali, R., Nehdi, A., Zahrani, H. A., Altolayyan, A., Shaibah, H., Baz, O., Alhallaj, A., Moresco, J. J., Diedrich, J. K., Yates, J. R., & Boudjelal, M. (2020). Proteomics profiling of kaimrc1 in comparison to MDA-MB231 and MCF-7. International journal of molecular sciences, 21(12), 1-15. [4328]. https://doi.org/10.3390/ijms21124328