Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use

Prerana Ramadurgum, John D. Hulleman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and accurate control of user-defined POIs via DDs, as well as the identification of novel (and potentially synergistic) small-molecule stabilizers. Here, we describe a platform for achieving these goals. For complete details on the use and execution of this protocol, please refer to Ramadurgum et al. (2020).

Original languageEnglish (US)
Article number100069
JournalSTAR Protocols
Volume1
Issue number2
DOIs
StatePublished - Sep 18 2020

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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