@article{e10908e0bc9648dc87ac79fd349d7f3e,
title = "Protocol for In Vivo Evaluation and Use of Destabilizing Domains in the Eye, Liver, and Beyond",
abstract = "Destabilizing domains (DDs) have been used successfully to conditionally control the abundance of proteins of interest (POIs) in a small-molecule-dependent manner in mice, worms (Caenorhabditis elegans), and Drosophila. However, development of such systems must account for delivery of the DD-POIs to the target tissue, accessibility of the target tissue to the small molecule, and quantification of stabilization. Here, we describe the considerations and steps to take in order to effectively implement a DD-POI in mouse ocular and hepatic tissue. For complete details on the use and execution of this protocol, please refer to Datta et al. (2018), Ramadurgum and Hulleman (2020), and Ramadurgum et al. (2020).",
author = "Prerana Ramadurgum and Steffi Daniel and Hulleman, {John D.}",
note = "Funding Information: We thank the following sources for providing the images used in the graphical abstract: mice image ( https://www.jax.org/news-and-insights/jax-blog/2013/may/the-difference-between-germ-free-and-specific-pathogen-free-mice ), fly image ( https://en.wikipedia.org/wiki/Drosophila_melanogaster , by Sanjay Acharya, own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=63862733 ), and worm image ( https://hymanlab.mpi-cbg.de/hyman_lab/c-elegans/ ). J.D.H. is supported by an endowment from the Roger and Dorothy Hirl Research Fund , a vision research grant from the Karl Kirchgessner Foundation , a National Eye Institute (NEI) R21 grant ( EY028261 ), an NEI R01 grant ( EY027785 ), and a Career Development award from RPB. Additional support was provided by an NEI Visual Science Core grant ( P30 EY030413 ) and an unrestricted grant from RPB (both to the UT Southwestern Department of Ophthalmology). Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = sep,
day = "18",
doi = "10.1016/j.xpro.2020.100094",
language = "English (US)",
volume = "1",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}