Protocol for sleep analysis in the brain of genetically modified adult mice

Kanako Iwasaki, Noriko Hotta-Hirashima, Hiromasa Funato, Masashi Yanagisawa

Research output: Contribution to journalArticlepeer-review

Abstract

Elucidating the molecular pathways that regulate animal behavior such as sleep is essential for understanding how the brain works. However, to examine how a certain functional domain of protein is involved in animal behavior is challenging. Here, we present a protocol for inducing endogenous protein that lacks a specific functional domain using Cre-mediated allele modification in neurons followed by electroencephalogram/electromyogram (EEG/EMG) recording to study the role of kinases in sleep. This strategy is applicable to other gene targets or behaviors. For complete details on the use and execution of this protocol, please refer to Iwasaki et al. (2021).

Original languageEnglish (US)
Article number100982
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - Dec 17 2021
Externally publishedYes

Keywords

  • Behavior
  • Model Organisms
  • Molecular Biology
  • Neuroscience

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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