Abstract
Sequential 1H and 15N assignments of human FKBP, a cytosolic binding protein for the immunosuppressive agents FK5O6 and rapamycin, are reported. A combination of homonuclear and relayed heteronuclear experiments has enabled assignment of 98 of 99 backbone amide NHs, 119 of 120 C°Hs, 97 of 99 non-proline amide 15Ns, and 375 of 412 side-chain resonances of this 107-residue protein. Long-range NOEs are used to demonstrate that FKBP has a novel folding topology consisting of a five-stranded antiparallel β sheet with +3, +1, −3, +1 loop connectivity.
Original language | English (US) |
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Pages (from-to) | 4774-4789 |
Number of pages | 16 |
Journal | Biochemistry |
Volume | 30 |
Issue number | 19 |
DOIs | |
State | Published - May 1 1991 |
ASJC Scopus subject areas
- Biochemistry