Proton and Nitrogen Sequential Assignments and Secondary Structure Determination of the Human FK5O6 and Rapamycin Binding Protein

Michael K. Rosen, Stephen W. Michnick, Martin Karplus, Stuart L. Schreiber

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Sequential 1H and 15N assignments of human FKBP, a cytosolic binding protein for the immunosuppressive agents FK5O6 and rapamycin, are reported. A combination of homonuclear and relayed heteronuclear experiments has enabled assignment of 98 of 99 backbone amide NHs, 119 of 120 C°Hs, 97 of 99 non-proline amide 15Ns, and 375 of 412 side-chain resonances of this 107-residue protein. Long-range NOEs are used to demonstrate that FKBP has a novel folding topology consisting of a five-stranded antiparallel β sheet with +3, +1, −3, +1 loop connectivity.

Original languageEnglish (US)
Pages (from-to)4774-4789
Number of pages16
JournalBiochemistry
Volume30
Issue number19
DOIs
StatePublished - May 1 1991

ASJC Scopus subject areas

  • Biochemistry

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