Proton relaxation studies in H2OD2O mixtures. The binding of manganese(II) to bovine serum albumin

A. Dean Sherry; Edward R. Birnbaum; Dennis W. Darnall

doi:10.1016/0003-2697(73)90044-4

Proton relaxation studies in H₂OD₂O mixtures. The binding of manganese(II) to bovine serum albumin

A. Dean Sherry, Edward R. Birnbaum, Dennis W. Darnall

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We have used the direct method for determining longitudinal relaxation times of water protons in H₂OD₂O mixtures. The relaxation time of pure water (2.7 sec) increases to 9.0 sec in 10% H₂O-90% D₂O mixture. This larger relaxation time enables us to use the direct method to accurately determine relaxation rate enhancements due to paramagnetic metal ions. The binding parameters for the interaction of manganese(II) to bovine serum albumin determined by this method are in excellent agreement with those determined earlier using pulsed methods.

Original language	English (US)
Pages (from-to)	415-420
Number of pages	6
Journal	Analytical biochemistry
Volume	52
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(73)90044-4
State	Published - Apr 1973

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Proton relaxation studies in H2OD2O mixtures. The binding of manganese(II) to bovine serum albumin",

abstract = "We have used the direct method for determining longitudinal relaxation times of water protons in H2OD2O mixtures. The relaxation time of pure water (2.7 sec) increases to 9.0 sec in 10% H2O-90% D2O mixture. This larger relaxation time enables us to use the direct method to accurately determine relaxation rate enhancements due to paramagnetic metal ions. The binding parameters for the interaction of manganese(II) to bovine serum albumin determined by this method are in excellent agreement with those determined earlier using pulsed methods.",

author = "Sherry, {A. Dean} and Birnbaum, {Edward R.} and Darnall, {Dennis W.}",

note = "Funding Information: This investigation was suporkd hs Grant, GB-31374 from the National Science Foundation. We also wish to ncknowledgc, n Sntional Sricxnre Foundation grant for th? purchns~ of the Jcolco PS-100 mlclear magnetic rcsonan~ qxctrometer.",

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doi = "10.1016/0003-2697(73)90044-4",

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T1 - Proton relaxation studies in H2OD2O mixtures. The binding of manganese(II) to bovine serum albumin

AU - Sherry, A. Dean

AU - Birnbaum, Edward R.

AU - Darnall, Dennis W.

N1 - Funding Information: This investigation was suporkd hs Grant, GB-31374 from the National Science Foundation. We also wish to ncknowledgc, n Sntional Sricxnre Foundation grant for th? purchns~ of the Jcolco PS-100 mlclear magnetic rcsonan~ qxctrometer.

PY - 1973/4

Y1 - 1973/4

N2 - We have used the direct method for determining longitudinal relaxation times of water protons in H2OD2O mixtures. The relaxation time of pure water (2.7 sec) increases to 9.0 sec in 10% H2O-90% D2O mixture. This larger relaxation time enables us to use the direct method to accurately determine relaxation rate enhancements due to paramagnetic metal ions. The binding parameters for the interaction of manganese(II) to bovine serum albumin determined by this method are in excellent agreement with those determined earlier using pulsed methods.

AB - We have used the direct method for determining longitudinal relaxation times of water protons in H2OD2O mixtures. The relaxation time of pure water (2.7 sec) increases to 9.0 sec in 10% H2O-90% D2O mixture. This larger relaxation time enables us to use the direct method to accurately determine relaxation rate enhancements due to paramagnetic metal ions. The binding parameters for the interaction of manganese(II) to bovine serum albumin determined by this method are in excellent agreement with those determined earlier using pulsed methods.

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Proton relaxation studies in H₂OD₂O mixtures. The binding of manganese(II) to bovine serum albumin

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