Proton relaxation studies in H2OD2O mixtures. The binding of manganese(II) to bovine serum albumin

A. Dean Sherry, Edward R. Birnbaum, Dennis W. Darnall

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have used the direct method for determining longitudinal relaxation times of water protons in H2OD2O mixtures. The relaxation time of pure water (2.7 sec) increases to 9.0 sec in 10% H2O-90% D2O mixture. This larger relaxation time enables us to use the direct method to accurately determine relaxation rate enhancements due to paramagnetic metal ions. The binding parameters for the interaction of manganese(II) to bovine serum albumin determined by this method are in excellent agreement with those determined earlier using pulsed methods.

Original languageEnglish (US)
Pages (from-to)415-420
Number of pages6
JournalAnalytical biochemistry
Volume52
Issue number2
DOIs
StatePublished - Apr 1973

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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