Proton spectral editing for discrimination of lactate and threonine 1.31 ppm resonances in human brain in vivo

Changho Choi, Nicholas J. Coupland, Sanjay Kalra, Paramjit P. Bhardwaj, Nikolai Malykhin, Peter S. Allen

Research output: Contribution to journalArticle

19 Scopus citations


A single-voxel proton NMR J-difference editing method for discriminating between the 1.31 ppm resonances of lactate (Lac) and threonine (Thr) in human brain in vivo at 3 T is reported. One double-band and two triple-band Gaussian 180° RF pulses, all with a bandwidth of 15 Hz, were employed within an adiabatic-refocused double-echo localization sequence to induce the target signals of Lac and Thr and simultaneously acquire a creatine singlet in each subscan. The optimum echo time and the editing efficiency were obtained by numerical analysis of the filtering performance. The Lac and Thr signals were extracted, without lipid contamination, from three subspectra. Using the calculated yields, the concentrations of Lac and Thr in the human occipital cortex were estimated to be 0.47 ± 0.07 and 0.55 ± 0.06 mM (mean ± SD, M = 7), respectively, with reference to Cr at 8 mM.

Original languageEnglish (US)
Pages (from-to)660-665
Number of pages6
JournalMagnetic Resonance in Medicine
Issue number3
Publication statusPublished - Sep 2006



  • Double-band and triple-band 180° RF pulses
  • Human brain
  • J-difference editing
  • Lactate
  • Threonine

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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