Proximal tubule dysfunction in cystine-loaded tubules: Effect of phosphate and metabolic substrates

Geeta Bajaj, Michel Baum

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Intracellular cys-tine loading by use of cystine dimethyl ester (CDME) results in a generalized inhibition in proximal tubule transport due, in part, to a decrease in intracellular ATP. The present study examined the importance of phosphate and metabolic substrates in the proximal tubule dysfunction produced by cystine loading. Proximal tubule intracellular phosphorus was 1.8 0.1 in control tubules and 1.1 0.1 nmol/mg protein in proximal tubules incubated in vitro with CDME (P 0.001). Infusion of sodium phosphate in rabbits and subsequent incubation of proximal tubules with a highphosphate medium attenuated the decrease in proximal tubule respiration and prevented the decrease in intracellular ATP with cystine loading. Tricarboxylic acid cycle intermediates have been shown to preserve oxidative metabolism in phosphate-depleted proximal tubules. In proximal tubules incubated with either 1 mM valerate or butyrate, there was a 42 and 34% reduction (both P 0.05) in the rate of oxygen consumption with cystine loading. However, tubules incubated with 1 mM succinate or citrate had only a 13 and 14% (P = NS) reduction in the rate of oxygen consumption, respectively. These data are consistent with a limitation of intracellular phosphate in the pathogenesis of the proximal tubule dysfunction with cystine loading.

Original languageEnglish (US)
Pages (from-to)F717-F722
JournalAmerican Journal of Physiology
Volume271
Issue number3 PART 2
StatePublished - Dec 1 1996

Keywords

  • Cystine dimethyl ester
  • Cystinosis
  • Fanconi syndrome

ASJC Scopus subject areas

  • Physiology (medical)

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