Pseudo-outbreak of Vancomycin-Resistant-Enterococcus (VRE) colonization in a neonatal intensive care unit using spectra VRE surveillance medium

Rita M. Gander, Dominick Cavuoti, Adnan Alatoom, Paul Southern, Debra Grant, Kathleen Salinas, Donna Gaffney, Jennifer MacKenzie, Linda Byrd

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

From November 2011 through March 2012, we surveyed 272 babies in our neonatal intensive care unit for rectal colonization with vancomycin-resistant enterococci (VRE). Using Spectra VRE medium (Remel Diagnostics, Lenexa, KS), we identified one neonate colonized with vancomycin-resistant Enterococcus faecium. In addition, 55 (13%) of the surveillance cultures yielded false-positive results with vancomycin-susceptible Enterococcus faecalis. During the same time period, 580 rectal swabs were collected from adult patients resulting in 20 (3%) false-positive cultures. The difference in false-positive rates between cultures from babies and adults was statistically significant (P>0.001), prompting an investigation of factors that might influence the elevated false-positive rate in the neonates including patient demographics, nutrition, and topical ointments applied at the time of testing. Older neonates, with a median age of 6 weeks, were more likely to have false-positive cultures than younger neonates with a median age of 3 weeks (P>0.001). The younger neonates receiving Similac Expert Care products were less likely to have false-positive surveillance cultures than those receiving other formulas (P>0.001). Application of topical products was not associated with false-positive cultures. The false-positive E. faecalis strains were typed by Diversilab Rep-PCR (bioMerieux, Marcy ĺEtoile, France) and found to represent eight different groups of isolates. The utility of the Spectra VRE media appeared to be significantly impacted by the age of the patients screened.

Original languageEnglish (US)
Pages (from-to)810-813
Number of pages4
JournalJournal of Clinical Microbiology
Volume51
Issue number3
DOIs
StatePublished - Mar 2013

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Neonatal Intensive Care Units
Disease Outbreaks
Newborn Infant
Enterococcus faecalis
Enterococcus faecium
Vancomycin
Ointments
France
Demography
Vancomycin-Resistant Enterococci
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

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Pseudo-outbreak of Vancomycin-Resistant-Enterococcus (VRE) colonization in a neonatal intensive care unit using spectra VRE surveillance medium. / Gander, Rita M.; Cavuoti, Dominick; Alatoom, Adnan; Southern, Paul; Grant, Debra; Salinas, Kathleen; Gaffney, Donna; MacKenzie, Jennifer; Byrd, Linda.

In: Journal of Clinical Microbiology, Vol. 51, No. 3, 03.2013, p. 810-813.

Research output: Contribution to journalArticle

Gander, Rita M. ; Cavuoti, Dominick ; Alatoom, Adnan ; Southern, Paul ; Grant, Debra ; Salinas, Kathleen ; Gaffney, Donna ; MacKenzie, Jennifer ; Byrd, Linda. / Pseudo-outbreak of Vancomycin-Resistant-Enterococcus (VRE) colonization in a neonatal intensive care unit using spectra VRE surveillance medium. In: Journal of Clinical Microbiology. 2013 ; Vol. 51, No. 3. pp. 810-813.
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