Pseudomonas aeruginosa corneal binding after 24-hour orthokeratology lens wear

Patrick M. Ladage, Nobutaka Yamamoto, Danielle M Robertson, James V. Jester, Walter M Petroll, Harrison D Cavanagh

Research output: Contribution to journalArticle

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Abstract

Purpose. To examine the effect of short-term 24-hr orthokeratology lens (OKL) wear on Pseudomonas aeruginosa binding, epithelial surface cell morphology, epithelial sheet thickness, and stromal thickness in a rabbit model. Methods. Seventeen New Zealand white rabbits were treated according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Partial membranectomy was performed on all rabbits 1 week before the experiments. Baseline values for epithelial and stromal thickness and epithelial surface cell size were determined by in vivo confocal microscopy in one randomly chosen eye (n = 6). One week later, rabbits were fitted in the same eye with a hyper oxygen-transmissible OKL. Twenty-four hours later, confocal microscopy was repeated. The second group of rabbits (n = 6) was fitted with an OKL in one randomly chosen eye for 24 hr. P. aeruginosa binding to the corneal epithelium was assessed for the control corneas and those exposed to the test lens. Scanning electron microscopy was performed on a third group of rabbits to assess epithelial surface damage (n = 5). Results. There was a statistically significant difference (P<0.001) in P. aeruginosa binding between the control (1.11 ± 0.74 × 105 colony-forming units per cornea) and the OKL-wearing eyes (2.74 ± 0.69 × 10 5 colony-forming units per cornea). The central epithelium thinned by 6.5% after lens wear (48.2 ± 1.9 μm to 45 ± 1.7 μm, P=0.005); however, central stromal thickness increased by 7.3% (322 ± 22 μm to 345 ± 29 μm, P=0.006). Compared with the baseline value, central epithelial cell size increased significantly from 1,253 ± 140 mm2 to 1,627 ± 393 mm2 (29.4%, P=0.02). Scanning electron microscopy showed increased surface epithelial damage associated with OKL wear. Conclusions. This prospective, masked, pilot study showed that 24-hr hyper oxygen-transmissible OKL wear induced a statistically significant increase in P. aeruginosa binding to the epithelium of the rabbit cornea, accompanied by central epithelial thinning, stromal thickening, and surface cell damage assessed by scanning electron microscopy. Collectively, the data suggest that despite adequate lens oxygen transmissibility, the mechanical pressure inherent in the OKL design exerted on the corneal surface appears to be associated with increased adherence of P. aeruginosa to surface corneal epithelial cells, which may pose an increased risk for lens-related microbial keratitis, especially in overnight (i.e., closed-eye) wearing conditions. Future studies are needed to determine whether these results are similar in human wear and how P. aeruginosa binding during OKL wear compares with other lens-wearing modalities, such as daily or continuous soft lens wear.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalEye and Contact Lens
Volume30
Issue number3
DOIs
StatePublished - Jul 2004

Fingerprint

Pseudomonas aeruginosa
Lenses
Rabbits
Cornea
Epithelial Cells
Electron Scanning Microscopy
Oxygen
Cell Size
Confocal Microscopy
Stem Cells
Epithelium
Corneal Epithelium
Crystalline Lens
Keratitis
Pressure

Keywords

  • Bacterial binding
  • Contact lenses
  • Corneal epithelium
  • Orthokeratology

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Pseudomonas aeruginosa corneal binding after 24-hour orthokeratology lens wear. / Ladage, Patrick M.; Yamamoto, Nobutaka; Robertson, Danielle M; Jester, James V.; Petroll, Walter M; Cavanagh, Harrison D.

In: Eye and Contact Lens, Vol. 30, No. 3, 07.2004, p. 173-178.

Research output: Contribution to journalArticle

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abstract = "Purpose. To examine the effect of short-term 24-hr orthokeratology lens (OKL) wear on Pseudomonas aeruginosa binding, epithelial surface cell morphology, epithelial sheet thickness, and stromal thickness in a rabbit model. Methods. Seventeen New Zealand white rabbits were treated according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Partial membranectomy was performed on all rabbits 1 week before the experiments. Baseline values for epithelial and stromal thickness and epithelial surface cell size were determined by in vivo confocal microscopy in one randomly chosen eye (n = 6). One week later, rabbits were fitted in the same eye with a hyper oxygen-transmissible OKL. Twenty-four hours later, confocal microscopy was repeated. The second group of rabbits (n = 6) was fitted with an OKL in one randomly chosen eye for 24 hr. P. aeruginosa binding to the corneal epithelium was assessed for the control corneas and those exposed to the test lens. Scanning electron microscopy was performed on a third group of rabbits to assess epithelial surface damage (n = 5). Results. There was a statistically significant difference (P<0.001) in P. aeruginosa binding between the control (1.11 ± 0.74 × 105 colony-forming units per cornea) and the OKL-wearing eyes (2.74 ± 0.69 × 10 5 colony-forming units per cornea). The central epithelium thinned by 6.5{\%} after lens wear (48.2 ± 1.9 μm to 45 ± 1.7 μm, P=0.005); however, central stromal thickness increased by 7.3{\%} (322 ± 22 μm to 345 ± 29 μm, P=0.006). Compared with the baseline value, central epithelial cell size increased significantly from 1,253 ± 140 mm2 to 1,627 ± 393 mm2 (29.4{\%}, P=0.02). Scanning electron microscopy showed increased surface epithelial damage associated with OKL wear. Conclusions. This prospective, masked, pilot study showed that 24-hr hyper oxygen-transmissible OKL wear induced a statistically significant increase in P. aeruginosa binding to the epithelium of the rabbit cornea, accompanied by central epithelial thinning, stromal thickening, and surface cell damage assessed by scanning electron microscopy. Collectively, the data suggest that despite adequate lens oxygen transmissibility, the mechanical pressure inherent in the OKL design exerted on the corneal surface appears to be associated with increased adherence of P. aeruginosa to surface corneal epithelial cells, which may pose an increased risk for lens-related microbial keratitis, especially in overnight (i.e., closed-eye) wearing conditions. Future studies are needed to determine whether these results are similar in human wear and how P. aeruginosa binding during OKL wear compares with other lens-wearing modalities, such as daily or continuous soft lens wear.",
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AU - Yamamoto, Nobutaka

AU - Robertson, Danielle M

AU - Jester, James V.

AU - Petroll, Walter M

AU - Cavanagh, Harrison D

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N2 - Purpose. To examine the effect of short-term 24-hr orthokeratology lens (OKL) wear on Pseudomonas aeruginosa binding, epithelial surface cell morphology, epithelial sheet thickness, and stromal thickness in a rabbit model. Methods. Seventeen New Zealand white rabbits were treated according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Partial membranectomy was performed on all rabbits 1 week before the experiments. Baseline values for epithelial and stromal thickness and epithelial surface cell size were determined by in vivo confocal microscopy in one randomly chosen eye (n = 6). One week later, rabbits were fitted in the same eye with a hyper oxygen-transmissible OKL. Twenty-four hours later, confocal microscopy was repeated. The second group of rabbits (n = 6) was fitted with an OKL in one randomly chosen eye for 24 hr. P. aeruginosa binding to the corneal epithelium was assessed for the control corneas and those exposed to the test lens. Scanning electron microscopy was performed on a third group of rabbits to assess epithelial surface damage (n = 5). Results. There was a statistically significant difference (P<0.001) in P. aeruginosa binding between the control (1.11 ± 0.74 × 105 colony-forming units per cornea) and the OKL-wearing eyes (2.74 ± 0.69 × 10 5 colony-forming units per cornea). The central epithelium thinned by 6.5% after lens wear (48.2 ± 1.9 μm to 45 ± 1.7 μm, P=0.005); however, central stromal thickness increased by 7.3% (322 ± 22 μm to 345 ± 29 μm, P=0.006). Compared with the baseline value, central epithelial cell size increased significantly from 1,253 ± 140 mm2 to 1,627 ± 393 mm2 (29.4%, P=0.02). Scanning electron microscopy showed increased surface epithelial damage associated with OKL wear. Conclusions. This prospective, masked, pilot study showed that 24-hr hyper oxygen-transmissible OKL wear induced a statistically significant increase in P. aeruginosa binding to the epithelium of the rabbit cornea, accompanied by central epithelial thinning, stromal thickening, and surface cell damage assessed by scanning electron microscopy. Collectively, the data suggest that despite adequate lens oxygen transmissibility, the mechanical pressure inherent in the OKL design exerted on the corneal surface appears to be associated with increased adherence of P. aeruginosa to surface corneal epithelial cells, which may pose an increased risk for lens-related microbial keratitis, especially in overnight (i.e., closed-eye) wearing conditions. Future studies are needed to determine whether these results are similar in human wear and how P. aeruginosa binding during OKL wear compares with other lens-wearing modalities, such as daily or continuous soft lens wear.

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KW - Contact lenses

KW - Corneal epithelium

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